Korean J Pediatr.  2004 Feb;47(2):204-209.

Effects of 7-Nitroindazole on Brain Cell Membrane Function and Energy Metabolism during Transient Global Cerebral Hypoxia-Ischemia and Reoxygenation-Reperfusion in Newborn Piglets

• Choi CW
• Hwang JH
• Chang YS
• Park WS

Abstract

PURPOSE
Our study was undertaken to discover whether a selective neuronal nitric oxide synthase inhibitor, 7-nitroindazole, influences brain cell membrane function and energy metabolism during and after transient global hypoxia-ischemia(HI) in newborn piglets.
METHODS
Cerebral HI was induced by temporary complete occlusion of bilateral common carotid arteries and simultaneous breathing with 8% oxygen for 30 minutes, followed by release of carotid occlusion and normoxic ventilation for one hour(reoxygenation-reperfusion, RR). 7-Nitroindazole(50 mg/kg) or vehicle was administered intraperitoneally just before the induction of HI or RR. Brain cortex was harvested for the biochemical analysis at the end of HI or RR.
RESULTS
The level of conjugated dienes significantly increased and the activity of Na+, K+-ATPase significantly decreased during HI, and they did not recover during RR. The levels of ATP and phosphocreatine(PCr) significantly decreased during HI, and recovered during RR. 7-Nitroindazole did not influence significantly the level of conjugated dienes, the activity of Na+, K+-ATPase, and the levels of ATP and PCr during HI and RR.
CONCLUSION
7-nitroindazole did not demonstrate a neuroprotective effect in our piglet model of transient global cerebral HI and one hour of RR.

Keyword

Hypoxia-ischemia; Brain; 7-Nitroindazole; Reperfusion injury; Metabolism; Animals; Newborn

MeSH Terms

Adenosine Triphosphate
Animals
Brain*
Carotid Artery, Common
Cell Membrane*
Energy Metabolism*
Humans
Hypoxia-Ischemia, Brain*
Infant, Newborn*
Metabolism
Neuroprotective Agents
Nitric Oxide Synthase Type I
Oxygen
Polymerase Chain Reaction
Reperfusion Injury
Respiration
Ventilation
Adenosine Triphosphate
Neuroprotective Agents
Nitric Oxide Synthase Type I
Oxygen