Korean J Otorhinolaryngol-Head Neck Surg.
2007 Nov;50(11):989-995.
Role of Toll-like Receptor 2 in a Mouse Model of Allergic Rhinitis
- Affiliations
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- 1Department of Otolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea. entkjm@catholic.ac.kr
Abstract
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BACKGROUND AND OBJECTIVES: Mammalian Toll-like receptors (TLR) participate in innate immune responses to microbial pathogens. Recent interest has been focused on the concept that TLR-induced innate responses can modulate subsequent adaptive immune responses. The objective of this study is to determine whether TLR 2 stimulation in vivo would modulate subsequent allergic responses in an Aspergillus fumigatus (Af) murine model of allergic rhinitis (AR).
MATERIALS AND METHOD: Mice were sensitized via intraperitoneal injection with Af antigen and alum, and received a series of three daily intranasal Af antigen challenge. A TLR 2 agonist, PamCys was administrated intranasally one day before sensitization or one day before the first intranasal allergen challenge. Adaptive immune profiles and response to Af challenge were assessed.
RESULTS
PamCys decreased the allergen induced nasal recruitment of eosinophils and interleukin (IL)-5 in nasal lavage fluids compared with mice treated with no PamCys. Serologic data revealed that PamCys downregulated Af-specific IgE in the sera of PamCys-treated mice. In addition, spleen cells from the PamCys-treated mice displayed attenuated Af-specific IL-4 and IL-5, but increased interferon (IFN)- and immunosuppressive IL-10.
CONCLUSION
The present study demonstrate that TLR 2 agonist decreases allergic responses in this AR model by shifting T helper 2 (Th2) biased immune parameters towards Th1 dominance.
MeSH Terms
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Animals
Aspergillus fumigatus
Bias (Epidemiology)
Eosinophils
Immunity, Innate
Immunoglobulin E
Injections, Intraperitoneal
Interferons
Interleukin-10
Interleukin-4
Interleukin-5
Interleukins
Mice*
Models, Animal
Nasal Lavage Fluid
Rhinitis*
Spleen
Toll-Like Receptor 2*
Toll-Like Receptors*
Immunoglobulin E
Interferons
Interleukin-10
Interleukin-4
Interleukin-5
Interleukins
Toll-Like Receptor 2
Toll-Like Receptors
