Immune Netw.  2007 Mar;7(1):26-30. 10.4110/in.2007.7.1.26.

Production of TNF-alpha and IL-6 in Macrophages by Mycobacterial Protein Antigens

• Ahn HJ
• Cho SN
• Paik TH
• Lee J
• Choi IH

• KMID: 2150624
• DOI: http://doi.org/10.4110/in.2007.7.1.26

Abstract

BACKGROUND: Mycobacterial antigens released as PIM, LM, LAM, lipoproteins and other cellular factors may contribute to macrophage and dendritic cell activation through pattern recognition receptors such as TLRs. In this study, we assessed cytokine production and ERK activation with stimulation of several major mycobacterial antigens.
METHODS
Purified mycobacterial antigens (10, 22, 30, 38kappaDa) and recombinant antigens (6, 16, 19, 38kappaDa, Ag85A antigen) were studied. The production of cytokines (TNF-alpha, IL-12, IL-6) was measured by ELISA. The ERK activation was detected by western blotting. The expression of TLR2 or TLR4 was measured by flow cytometry.
RESULTS
Among purified antigens only 30kappaDa antigen induced production of IL-6 or TNF-alpha in THP-1 macrophage cells. When THP-1 macrophage cells were treated with 30kappaDa antigen, phosphorylation of ERK was detected. ERK activation also occurred in TLR2 transfectant HEK293 cells with 30kappaDa antigen stimulation.
CONCLUSION
30kappaDa antigen is one of the major mycobacterial antigens inducing cytokine production and MAP kinases phosphorylation in macrophages.

Keyword

Macrophage; M. tuberculosis; 30kappaDa antigen; TNF-alpha; IL-6; ERK

MeSH Terms

Blotting, Western
Cytokines
Dendritic Cells
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
HEK293 Cells
Interleukin-12
Interleukin-6*
Lipoproteins
Macrophages*
Phosphorylation
Phosphotransferases
Receptors, Pattern Recognition
Tumor Necrosis Factor-alpha*
Cytokines
Interleukin-12
Interleukin-6
Lipoproteins
Phosphotransferases
Receptors, Pattern Recognition
Tumor Necrosis Factor-alpha