Korean J Infect Dis.  1998 Dec;30(6):499-506.

Determination of Genetic Subtypes of HIV- 1 Isolated from Korean Patients

Affiliations
  • 1Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.
  • 2Department of Clinical Pathology, Seoul National University College of Medicine, Seoul, Korea.
  • 3Clinical Research Institute, Seoul National University Hospital, Seoul, Korea.

Abstract

BACKGROUND
Limited data are available on genetic subtypes of HIV- 1 in Korean AIDS patients. To determine subtypes of HIV-1 in Korean patients, we analyzed nucleotide sequences of the env gene of HIV-1 and constructed a phylogenetic tree.
METHODS
Nineteen patients infected with HIV-1 were enrolled. The median CD4 + count was 85/mm 3. Peripheral blood mononuclear cells (PBMC) were collected and co-cultivated with pre-stimulated PBMC from HIV-seronegative donors for 7 ~14 days. DNA was extracted from cultured lymphocytes and proviral V3 region of the env gene was amplified by nested polymerase chain reaction (PCR) for direct sequencing without cloning. Sequence analysis was performed by cycle- sequencing and dye terminator methods with an automated DNA Sequenator. The sequences were aligned with nines ets of reference sequences for each subtype by Clustal method. Phylogenetic tree was constructed by the neighbor j oining method.
RESULTS
Eighteen of the nineteen sequences fell into subtype B (95%) and one was subtype A (5%). The patient nfected with subtype A was an ex-prostitute and had engaged in sexual contact with sailors who are generally regarded as one of the highest risk groups of HIV infection in Korea. The tetrameric motifs at the tip of the V3 loop were comprised of GPGR (six cases, 32%), GPGS (three cases, 16%), GPGQ, GPGG, GPGK, APGS (one case each, 5%)
CONCLUSION
Subtype B is predominant clade of HIV-1 isolated from Korean patients and only one case showed subtype A.

Keyword

HIV-1; Subtype; Phylogeny; env; V3

MeSH Terms

Base Sequence
Clone Cells
Cloning, Organism
DNA
Genes, env
HIV Infections
HIV-1
Humans
Korea
Lymphocytes
Military Personnel
Phylogeny
Polymerase Chain Reaction
Sequence Analysis
Tissue Donors
DNA
Full Text Links
  • KJID
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2022 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr