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Molecular Genetic and Serologic Analysis of the O allele in the Korean Population

Lee JY, Song SA, Oh SH

BACKGROUND: The recent expansion of knowledge about various ABO alleles has led to the need for a comprehensive measure to cover the numerous polymorphisms dispersed in the ABO gene. A...
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Transient expression of hemagglutinin antigen from canine influenza virus H3N2 in Nicotiana benthamiana and Lactuca sativa

Maharjan P, Choe S

PURPOSE: Canine influenza virus (CIV), H3N2, carries potentiality for zoonotic transmission and genetic assortment which raises a concern on possible epidemics, and human threats in future. To manage possible threats,...
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BRCA1 and Breast Cancer: a Review of the Underlying Mechanisms Resulting in the Tissue-Specific Tumorigenesis in Mutation Carriers

Semmler L, Reiter-Brennan C, Klein A

Since the first cloning of BRCA1 in 1994, many of its cellular interactions have been elucidated. However, its highly specific role in tumorigenesis in the breast tissue—carriers of BRCA1 mutations...
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A Journey to Understand Glucose Homeostasis: Starting from Rat Glucose Transporter Type 2 Promoter Cloning to Hyperglycemia

Ahn YH

My professional journey to understand the glucose homeostasis began in the 1990s, starting from cloning of the promoter region of glucose transporter type 2 (GLUT2) gene that led us to...
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An Outbreak of Histomoniasis in Backyard Sanhuang Chickens

Liu D, Kong L, Tao J, Xu J

Histomonas meleagridis is a facultative anaerobic parasite, which can cause a common poultry disease known as histomoniasis. The species and age of the birds impacts on the susceptibility, with turkey...
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Primary Myelofibrosis with MPL S505N Mutation: The First Case Reported in Korea

Kim UJ, Lee HJ, Choi IS, Kang SH, Jang SJ, Moon DS, Park G

MPL mutation is an important molecular marker in myeloproliferative neoplasms (MPN). Although MPL W515 is a hot spot for missense mutations in MPN, MPL S505 mutations have been reported in...
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Regulation of Osteoblast Metabolism by Wnt Signaling

Moorer MC, Riddle RC

Wnt/β-catenin signaling plays a critical role in the achievement of peak bone mass, affecting the commitment of mesenchymal progenitors to the osteoblast lineage and the anabolic capacity of osteoblasts depositing...
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Health and temperaments of cloned working dogs

Kim MJ, Oh HJ, Hwang SY, Hur TY, Lee BC

Dogs serve human society in various ways by working at tasks that are based on their superior olfactory sensitivity. However, it has been reported that only about half of all...
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Genetic Diversity of Echinococcus granulosus Genotype G1 in Xinjiang, Northwest of China

Yan B, Liu X, Wu J, Zhao S, Yuan W, Wang B, Wureli H, Tu C, Chen C, wang Y

Cystic echinococcosis (CE) caused by E. granulosus is a serious helminthic zoonosis in humans, livestock and wildlife. Xinjiang is one of high endemic province for CE in China. A total...
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The Effect of Recombinant Tyrosine Hydroxylase Expression on the Neurogenic Differentiation Potency of Mesenchymal Stem Cells

Turac G, Duruksu G, Karaoz E

OBJECTIVE: Tyrosine hydroxylase (TH) is a rate-limiting enzyme in dopamine synthesis, making the enhancement of its activity a target for ensuring sufficient dopamine levels. Rat bone marrow mesenchymal stem cells...
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Variable localization of Toll-like receptors in human fallopian tube epithelial cells

Amjadi F, Zandieh Z, Salehi E, Jafari R, Ghasemi N, Aflatoonian A, Fazeli A, Aflatoonian R

OBJECTIVE: To determine the localization, expression, and function of Toll-like receptors (TLRs) in fallopian tube epithelial cells. METHODS: The localization of TLRs in fallopian tube epithelial cells was investigated by immunostaining....
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Influenza M1 Virus-Like Particles Consisting of Toxoplasma gondii Rhoptry Protein 4

Lee SH, Lee DH, Piao Y, Moon EK, Quan FS

Toxoplasma gondii infections occur throughout the world, and efforts are needed to develop various vaccine candidates expressing recombinant protein antigens. In this study, influenza matrix protein (M1) virus-like particles (VLPs)...
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Next-generation sequencing enables the discovery of more diverse positive clones from a phage-displayed antibody library

Yang W, Yoon A, Lee S, Kim S, Han J, Chung J

Phage display technology provides a powerful tool to screen a library for a binding molecule via an enrichment process. It has been adopted as a critical technology in the development...
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Sphingosine 1-Phosphate Receptor Modulators and Drug Discovery

Park SJ, Im DS

Initial discovery on sphingosine 1-phosphate (S1P) as an intracellular second messenger was faced unexpectedly with roles of S1P as a first messenger, which subsequently resulted in cloning of its G...
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Learning, memory and exploratory similarities in genetically identical cloned dogs

Shin CW, Kim GA, Park WJ, Park KY, Jeon JM, Oh HJ, Kim MJ, Lee BC

Somatic cell nuclear transfer allows generation of genetically identical animals using donor cells derived from animals with particular traits. To date, few studies have investigated whether or not these cloned...
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Reproductive ability of a cloned male detector dog and behavioral traits of its offspring

Lee JH, Kim GA, Kim RS, Lee JS, Oh HJ, Kim MJ, Hong DK, Lee BC

In 2007, seven detector dogs were produced by somatic cell nuclear transfer using one nuclear donor dog, then trained and certified as excellent detector dogs, similar to their donor. In...
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A polymorphic minisatellite region of BORIS regulates gene expression and its rare variants correlate with lung cancer susceptibility

Yoon SL, Roh YG, Chu IS, Heo J, Kim SI, Chang H, Kang TH, Chung JW, Koh SS, Larionov V, Leem SH

Aberrant expression of BORIS/CTCFL (Brother of the Regulator of Imprinted Sites/CTCF-like protein) is reported in different malignancies. In this study, we characterized the entire promoter region of BORIS/CTCFL, including the...
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Improved development of somatic cell cloned bovine embryos by a mammary gland epithelia cells in vitro model

He XY, Ma LB, He XN, Si WT, Zheng YM

Previous studies have established a bovine mammary gland epithelia cells in vitro model by the adenovirus-mediated telomerase (hTERT-bMGEs). The present study was conducted to confirm whether hTERT-bMGEs were effective target...
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Construction of a New T-Vector: Nickase (Nt.BspQI)-Generated T-Vector Bearing a Reddish-Orange Indicator Gene

Choi JY, Jo C, Jo SA

T-vectors are widely used for cloning the polymerase chain reaction (PCR) products. However, the low conversion efficiency of a plasmid into the linear T-vector usually results in non-recombinants. Here, we...
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In silico analysis of Brucella abortus Omp2b and in vitro expression of SOmp2b

Golshani M, Vaeznia N, Sahmani M, Bouzari S

PURPOSE: At present, there is no vaccine available for the prevention of human brucellosis. Brucella outer membrane protein 2b (Omp2b) is a 36 kD porin existed in common Brucella pathogens...
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