J Lipid Atheroscler.  2020 Jan;9(1):184-194. 10.12997/jla.2020.9.1.184.

Curcumin Attenuates Acrolein-induced COX-2 Expression and Prostaglandin Production in Human Umbilical Vein Endothelial Cells

Affiliations
  • 1Department of Microbiology, College of Medicine, Kyung Hee University, Seoul, Korea. yongseek@khu.ac.kr
  • 2Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, Korea.

Abstract


OBJECTIVE
Inflammation is crucial to limiting vascular disease. Previously we reported that acrolein, a known toxin in tobacco smoke, might play an important role in the progression of atherosclerosis via an inflammatory response involving cyclooxygenase-2 (COX-2) and prostaglandin production in human umbilical vein endothelial cells (HUVECs). Curcumin has been known to improve vascular function and have anti-inflammatory properties. In this study, we investigated whether curcumin prevents the induction of inflammatory response caused by acrolein.
METHODS
Anti-inflammatory effects of curcumin were examined in acrolein-stimulated HUVECs. Induction of proteins, mRNA, prostaglandin and reactive oxygen species (ROS) were measured using immunoblot analysis, real-time reverse-transcription polymerase chain reaction, enzyme-linked immunosorbent assay and flow cytometry, respectively.
RESULTS
Curcumin attenuates inflammatory response via inhibition of COX-2 expression and prostaglandin production in acrolein-induced human endothelial cells. This inhibition by curcumin results in the abolition of phosphorylation of protein kinase C, p38 mitogen-activated protein kinase, and cAMP response element-binding protein. Furthermore, curcumin suppresses the production of ROS and endoplasmic reticulum stress via phosphorylation of eukaryotic initiation factor-2α caused by acrolein.
CONCLUSION
These results suggest that curcumin might be a useful agent against endothelial dysfunction caused by acrolein-induced inflammatory response.

Keyword

Inflammation; Acrolein; Curcumin; Cyclooxygenase-2; Human umbilical vein endothelial cells

MeSH Terms

Acrolein
Atherosclerosis
Curcumin*
Cyclic AMP Response Element-Binding Protein
Cyclooxygenase 2
Endoplasmic Reticulum Stress
Endothelial Cells
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Human Umbilical Vein Endothelial Cells*
Humans*
Inflammation
Phosphorylation
Polymerase Chain Reaction
Protein Kinase C
Protein Kinases
Reactive Oxygen Species
RNA, Messenger
Smoke
Tobacco
Vascular Diseases
Acrolein
Curcumin
Cyclic AMP Response Element-Binding Protein
Cyclooxygenase 2
Protein Kinase C
Protein Kinases
RNA, Messenger
Reactive Oxygen Species
Smoke
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