Ann Lab Med.  2020 Mar;40(2):155-163. 10.3343/alm.2020.40.2.155.

Upregulated Long Noncoding RNA LINC01234 Predicts Unfavorable Prognosis for Colorectal Cancer and Negatively Correlates With KLF6 Expression

Affiliations
  • 1Department of Ultrasound, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu, China. zzqqyy88@163.com

Abstract

BACKGROUND
LINC01234, a long noncoding RNA (lncRNA), is overexpressed in several cancers, including colorectal cancer (CRC). We investigated the role of LINC01234 in CRC development and confirmed its correlation with Krüppel-like factor 6 (KLF6), a tumor suppressor gene that is dysregulated in CRC.
METHODS
We tested mRNA levels using quantitative reverse transcription PCR (qRT-PCR). Tissue samples from patients with CRC, inflammatory bowel disease (IBD), hyperplastic polyp, and adenoma were included. Correlations between clinicopathological parameters, overall survival (OS) rate, and LINC01234 were analyzed using Kruskal-Wallis H test. Additionally, cell proliferation, apoptosis, and tumor formation in nude mice were tested to investigate the mechanism of LINC01234. Western blotting was used to determine protein levels.
RESULTS
LINC01234 expression was significantly upregulated in CRC tissues and CRC cell lines than in non-tumor tissues and normal epithelial cells, respectively. LINC01234 was associated with high tumor stage, larger tumor size, and metastasis. Patients with higher LINC01234 expression showed reduced OS. Cell proliferation was inhibited by LINC01234 knockdown, whereas apoptosis was enhanced. Mice injected with SW480 cells with LINC01234 knockdown displayed decreased tumor volume, weight, and Ki-67 levels compared with those injected with control cells. KLF6 was negatively regulated by LINC01234. Overexpression of KLF6 showed effects similar to those observed following LINC01234 knockdown on cell proliferation and apoptosis.
CONCLUSIONS
LINC01234 could be a prognostic biomarker in CRC patients. Upregulation of LINC01234 in CRC promotes tumor development through negative regulation of KLF6.

Keyword

Long noncoding RNA; LINC01234; Colorectal cancer; Krüppel-like factor 6; Prognosis

MeSH Terms

Adenoma
Animals
Apoptosis
Blotting, Western
Cell Line
Cell Proliferation
Colorectal Neoplasms*
Epithelial Cells
Genes, Tumor Suppressor
Humans
Inflammatory Bowel Diseases
Mice
Mice, Nude
Neoplasm Metastasis
Polymerase Chain Reaction
Polyps
Prognosis*
Reverse Transcription
RNA, Long Noncoding*
RNA, Messenger
Tumor Burden
Up-Regulation
RNA, Long Noncoding
RNA, Messenger
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