Lab Anim Res.  2016 Dec;32(4):194-199. 10.5625/lar.2016.32.4.194.

Ischemic brain injury decreases dynamin-like protein 1 expression in a middle cerebral artery occlusion animal model and glutamate-exposed HT22 cells

Affiliations
  • 1Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, Jinju, Korea. pokoh@gnu.ac.kr

Abstract

Dynamin-like protein I (DLP-1) is an important mitochondrial fission and fusion protein that is associated with apoptotic cell death in neurodegenerative diseases. In this study, we investigated DLP-1 expression in a focal cerebral ischemia animal model and glutamate-exposed hippocampal-derived cell line. Middle cerebral artery occlusion (MCAO) was surgically induced in adult male rats to induce focal cerebral ischemic injury. Brain tissues were collected 24 hours after the onset of MCAO. MCAO induces an increase in infarct volume and histopathological changes in the cerebral cortex. We identified a decrease in DLP-1 in the cerebral cortices of MCAO-injured animals using a proteomic approach and Western blot analysis. Moreover, glutamate treatment significantly decreased DLP-1 expression in a hippocampal-derived cell line. The decrease in DLP-1 indicates mitochondrial dysfunction. Thus, these results suggest that neuronal cell injury induces a decrease in DLP-1 levels and consequently leads to neuronal cell death.

Keyword

Brain ischemia; MCAO; dynamin-like protein 1; hippocampal-derived cell line

MeSH Terms

Adult
Animals
Blotting, Western
Brain Injuries*
Brain Ischemia
Brain*
Cell Death
Cell Line
Cerebral Cortex
Glutamic Acid
Humans
Infarction, Middle Cerebral Artery*
Male
Middle Cerebral Artery*
Mitochondrial Dynamics
Models, Animal
Neurodegenerative Diseases
Neurons
Rats
Glutamic Acid
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