Lab Anim Res.  2015 Dec;31(4):198-203. 10.5625/lar.2015.31.4.198.

Curcumin attenuates the middle cerebral artery occlusion-induced reduction in gamma-enolase expression in an animal model

• Gim SA
• Lee SR
• Shah FA
• Koh PO

• KMID: 2312146
• DOI: http://doi.org/10.5625/lar.2015.31.4.198

Abstract

Curcumin exerts a protective effect in cerebral ischemia through its anti-oxidant and anti-inflammatory activities. gamma-enolase is a glycolytic enzyme expressed in neurons that is known to exerts a neuroprotective effect. We investigated whether curcumin regulates gamma-enolase expression in focal cerebral ischemic injury in rats. Middle cerebral artery occlusion (MCAO) was performed to induce focal cerebral ischemia. Adult male rats were injected intraperitoneally with either vehicle or curcumin (50 mg/kg) 1 h after MCAO and cerebral cortex tissues were isolated 24 h after MCAO. We found that MCAO-induced injury resulted in a reduction in gamma-enolase expression in vehicle-treated animals using a proteomics approach. However, this reduction was attenuated in animals with MCAO treated with curcumin. Reverse-transcription PCR and Western blot analyses also showed that curcumin treatment prevented the MCAO injury-induced reduction in gamma-enolase expression. The results of this study suggest that curcumin exerts its neuroprotective function in focal cerebral ischemia by regulating the expression of gamma-enolase.

Keyword

gamma-enolase; curcumin; neuroprotection

MeSH Terms

Adult
Animals*
Blotting, Western
Brain Ischemia
Cerebral Cortex
Curcumin*
Humans
Infarction, Middle Cerebral Artery
Male
Middle Cerebral Artery*
Models, Animal*
Neurons
Neuroprotective Agents
Phosphopyruvate Hydratase*
Polymerase Chain Reaction
Proteomics
Rats
Curcumin
Neuroprotective Agents
Phosphopyruvate Hydratase

Figure

• 

  Figure 1 γ-Enolase protein spots identified by MALDI-TOF in the cerebral cortices from vehicle+sham, curcumin+sham, vehicle+middle cerebral artery occlusion (MCAO), and curcumin+MCAO animals. Squares indicate the protein spots. The intensity of spots was measured using PDQuest software. The ratio of intensity is described as spots intensity of these animals to spots intensity of sham+vehicle animals. Data are shown as mean±S.E.M. *P<0.05.

• 

  Figure 2 Reverse transcription-PCR analysis of γ-enolase in the cerebral cortices from vehicle+sham, curcumin+sham, vehicle+middle cerebral artery occlusion (MCAO), and curcumin+MCAO animals. Each lane represents an individual experimental animal. Densitometric analysis is represented as intensity of γ-enolase to intensity of actin. Data (n=5) are represented as mean±S.E.M. *P<0.05.

• 

  Figure 3 Western blot analysis of γ-enolase in the cerebral cortices from vehicle+sham, curcumin+sham, vehicle+middle cerebral artery occlusion (MCAO), and curcumin+MCAO animals. Each lane represents an individual experimental animal. Densitometric analysis is represented as intensity of g-enolase to intensity of actin. Data (n=5) are represented as mean±S.E.M. *P<0.05.

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