Lab Anim Res.  2015 Sep;31(3):134-138. 10.5625/lar.2015.31.3.134.

Curcumin treatment recovery the decrease of protein phosphatase 2A subunit B induced by focal cerebral ischemia in Sprague-Dawley rats

• Shah FA
• Park DJ
• Gim SA
• Koh PO

• KMID: 2312130
• DOI: http://doi.org/10.5625/lar.2015.31.3.134

Abstract

Curcumin provides various biological effects through its anti-inflammatory and antioxidant properties. Moreover, curcumin exerts a neuroprotective effect against ischemic condition-induced brain damage. Protein phosphatase 2A (PP2A) is a ubiquitous serine and threonine phosphatase with various cell functions and broad substrate specificity. Especially PP2A subunit B plays an important role in nervous system. This study investigated whether curcumin regulates PP2A subunit B expression in focal cerebral ischemia. Cerebral ischemia was induced surgically by middle cerebral artery occlusion (MCAO). Adult male rats were injected with either vehicle or curcumin (50 mg/kg) 1 h after MCAO and cerebral cortex tissues were isolated 24 h after MCAO. A proteomics study, reverse transverse-PCR and Western blot analyses were performed to examine PP2A subunit B expression levels. We identified a reduction in PP2A subunit B expression in MCAO-operated animals using a proteomic approach. However, curcumin treatment prevented injury-induced reductions in PP2A subunit B levels. Reverse transverse-PCR and Western blot analyses confirmed that curcumin treatment attenuated the injury-induced reduction in PP2A subunit B levels. These findings can suggest that the possibility that curcumin maintains levels of PP2A subunit B in response to cerebral ischemia, which likely contributes to the neuroprotective function of curcumin in cerebral ischemic injury.

Keyword

Curcumin; neuroprotection; protein phosphatase 2A subunit B

MeSH Terms

Adult
Animals
Blotting, Western
Brain
Brain Ischemia*
Cerebral Cortex
Curcumin*
Humans
Infarction, Middle Cerebral Artery
Male
Nervous System
Neuroprotective Agents
Phosphoprotein Phosphatases
Protein Phosphatase 2*
Proteomics
Rats
Rats, Sprague-Dawley*
Serine
Substrate Specificity
Curcumin
Neuroprotective Agents
Phosphoprotein Phosphatases
Protein Phosphatase 2
Serine

Figure

• 

  Figure 1 Protein phosphatase 2A (PP2A) subunit B protein spots identified by MALDI-TOF in the cerebral cortices from vehicle+sham, curcumin+sham, vehicle+middle cerebral artery occlusion (MCAO), and curcumin+MCAO animals. Squares indicate the protein spots. The intensity of spots was measured using PDQuest software. The ratio of intensity is described as spots intensity of these animals to spots intensity of sham+vehicle animals. Data (n=3) are shown as mean±S.E.M. *P<0.05.

• 

  Figure 2 Reverse transverse-PCR analysis of protein phosphatase 2A (PP2A) subunit B in the cerebral cortices from vehicle+sham, curcumin+sham, vehicle+middle cerebral artery occlusion (MCAO), and curcumin+MCAO animals. Each lane represents an individual experimental animal. Densitometric analysis is represented as intensity of PP2A subunit B to intensity of actin. Data (n=5) are represented as mean±S.E.M. *P<0.05.

• 

  Figure 3 Western blot analysis of protein phosphatase 2A (PP2A) subunit B in the cerebral cortices from vehicle+sham, curcumin+sham, vehicle+middle cerebral artery occlusion (MCAO), and curcumin+MCAO animals. Each lane represents an individual experimental animal. Densitometric analysis is represented as intensity of PP2A subunit B to intensity of actin. Data (n=5) are represented mean±S.E.M. *P<0.05.

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