Clin Exp Otorhinolaryngol.  2011 Dec;4(4):163-167.

Clinical Approaches for Understanding the Expression Levels of Pattern Recognition Receptors in Otitis Media with Effusion

• Lee SY
• Ryu EW
• Kim JB
• Yeo SG

Abstract

OBJECTIVES
Bacterial infections in the normally sterile environment of the middle ear cavity usually trigger host immune response, whereby the innate immune system plays a dominant role as the host's first line of defense. We evaluated the expression levels of Toll-like receptors (TLRs) -2, -4, -5, -9, and nucleotide-binding oligomerization domain-containing proteins (NODs) -1 and -2, all of which are related to bacterial infection in pediatric patients with otitis media with effusion (OME).
METHODS
The study sample consisted of 46 pediatric patients with OME, all of whom had ventilation tubes inserted. The expression levels of TLR-2, -4, -5, -9, NOD-1 and -2 mRNA in middle ear effusion were assessed by polymerase chain reaction (PCR). Difference of pattern recognition receptors (PRRs) expression level by presence of bacteria, ventilation tube insertion rate, and effusion fluid character was assessed.
RESULTS
All effusion fluid samples collected from patients with OME showed expression of TLR-2, -4, -5, -9, NOD-1, and -2 mRNA by PCR. However, we found no differences among expression levels of PRRs in relation to characteristics of exudates, presence of bacteria, or frequencies of ventilation tube insertion (P>0.05).
CONCLUSION
Our findings suggest that exudates of OME patients show PRR expressions that are related to the innate immune response regardless of the characteristics of effusion fluid, presence of bacteria in exudates, or frequency of ventilation tube insertion.

Keyword

Otitis media with effusion; Pattern recognition receptors; Innate immunity

MeSH Terms

Bacteria
Bacterial Infections
Ear, Middle
Exudates and Transudates
Humans
Immune System
Immunity, Innate
Otitis
Otitis Media
Otitis Media with Effusion
Polymerase Chain Reaction
Porcine Reproductive and Respiratory Syndrome
Proteins
Receptors, Pattern Recognition
RNA, Messenger
Toll-Like Receptors
Ventilation
Proteins
RNA, Messenger
Receptors, Pattern Recognition
Toll-Like Receptors

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