Clin Exp Otorhinolaryngol.  2014 Mar;7(1):7-12.

Decreased Pattern-Recognition Receptor-Mediated Cytokine mRNA Expression in Obese Children With Otitis Media With Effusion

Affiliations
  • 1Kyung Hee University College of Nursing Science, Seoul, Korea.
  • 2Department of Pediatrics, Kyung Hee University School of Medicine, Seoul, Korea.
  • 3Department of Otolaryngology, Kyung Hee University School of Medicine, Seoul, Korea. yeo2park@gmail.com
  • 4Medical Science Research Institute, Kyung Hee University Medical Center, Seoul, Korea.

Abstract


OBJECTIVES
To assess innate and humoral immune responses in middle ear effusion of obese pediatric patients with otitis media with effusion (OME).
METHODS
We evaluated 219 children with OME, of whom 21 were obese and 198 were non-obese. We compared the expression in middle ear effusion of mRNAs encoding toll-like receptors (TLR) 2, 4, 5, and 9; nucleotide-binding oligomerization domains (NOD) 1 and 2; retinoic acid-inducible gene (RIG)-I; interleukins (IL)-6, -10, and -12; interferon (IFN)-gamma; and tumor necrosis factor (TNF)-alpha mRNAs. We also compared the expression of immunoglobulins IgG, IgA, and IgM and the bacterial detection rate in the two groups.
RESULTS
TLR2-mediated expression of IL-6 mRNA, TLR4-mediated expression of IL-6 and IL-10 mRNA, TLR5-mediated expression of IL-6, IL-10, and TNF-alpha mRNA, TLR9-mediated expression of IL-6 mRNA, and NOD2-mediated expression of IL-6, IL-12, and TNF-alpha mRNA were significantly lower in obese than in non-obese children (P<0.05). However, concentrations of IgG, IgA, and IgM in middle ear effusion were lower in obese than in non-obese children, but none of these differences was significant (P>0.05).
CONCLUSION
Mean body mass index was higher and pattern-recognition receptor-mediated cytokine mRNA expression was lower in obese than in non-obese children with OME.

Keyword

Otitis media; Obesity; Pattern-recognition receptors; Immunoglobulin; Immunity; Bacteria

MeSH Terms

Bacteria
Body Mass Index
Child*
Humans
Immunity, Humoral
Immunoglobulin A
Immunoglobulin G
Immunoglobulin M
Immunoglobulins
Interferons
Interleukin-10
Interleukin-12
Interleukin-6
Interleukins
Obesity
Otitis Media with Effusion*
Otitis Media*
Otitis*
RNA, Messenger*
Toll-Like Receptors
Tumor Necrosis Factor-alpha
Immunoglobulin A
Immunoglobulin G
Immunoglobulin M
Immunoglobulins
Interferons
Interleukin-10
Interleukin-12
Interleukin-6
Interleukins
RNA, Messenger
Toll-Like Receptors
Tumor Necrosis Factor-alpha

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