Lab Anim Res.  2010 Dec;26(4):385-391. 10.5625/lar.2010.26.4.385.

Biological Activity and Single Oral Dose Toxicity of Dansam-samultang

• Lee IC
• Kim BH
• Kim MK

• KMID: 2114707
• DOI: http://doi.org/10.5625/lar.2010.26.4.385

Abstract

In this study, we investigated the in vitro antioxidative effects, antimicrobial activities and single oral dose toxicity of the extracts from Dansam-samultang to evaluate its use as a functional ingredient in cosmetics. In the antioxidative effect, the ethanol extract from Dansam-samultang (DSE) had higher antioxidant values of 92.0% at 1,000 microg/mL than that of water extract from Dansam-samultang (DSW, 86.0%) when evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. The superoxide dismutase-like activity of DSW and DSE were 16.3% and 21.3% at 1,000 microg/mL in concentration, respectively. Xanthine oxidase inhibition activity of the DSE was higher 51.5% than that of the DSW (21.4%). This study was also undertaken to test the in vitro antimicrobial activity with the extracts of Dansam-samultang. In general, the DSE showed the significant antimicrobial activity against Staphylococcus aureus, Staphylococcus epidermiders and Escherichia coli. In single oral dose toxicity study, in vivo, there were no differences between control and treated groups in clinical signs, body weight gains, and gross finding. The results indicated that DSE did not show any toxic effects at 10 mL/kg in mice, and the LD50 of DSE was found to be higher than 10 mL/kg in this experiment. In conclusion, the extracts from Dansam-samultang may act as a natural subsistence for functional cosmetics.

Keyword

Dansam-samultang; antioxidative activity; antimicrobial activity; single oral dose toxicity

MeSH Terms

Animals
Biphenyl Compounds
Body Weight
Cosmetics
Escherichia coli
Ethanol
Lethal Dose 50
Mice
Picrates
Staphylococcus
Staphylococcus aureus
Superoxides
Water
Xanthine Oxidase
Biphenyl Compounds
Cosmetics
Ethanol
Picrates
Superoxides
Water
Xanthine Oxidase

Figure

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  Figure 1. The procedure for extraction from Dansam-samultang.

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  Figure 2. Antioxidative effect of Dansam-samultang extracts on DPPH radical scavenging activity. DSW, Dansam-samultang water extracts; DSE, Dansam-samultang ethanol extracts. The values are means 3 replicates and those with different alphabet letters are significantly different at P<0.05.

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  Figure 3. SOD-like activity of Dansam-samultang extracts. DSW, Dansam- samultang water extracts; DSE, Dansam-samultang ethanol extracts. The values are means 3 replicates and those with different alphabet letters are significantly different at P<0.05.

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  Figure 4. Inhibition rate of Dansam-samultang extracts on xanthine oxidase. DSW, Dansam-samultang water extracts; DSE, Dansam-samultang ethanol extracts. The values are means 3 replicates and those with different alphabet letters are significantly different at P<0.05.

Reference

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