Lab Anim Res.  2010 Mar;26(1):15-20. 10.5625/lar.2010.26.1.15.

Investigations of Samwhang-sasimtang Extracts on Biological Activities In Vitro and Vivo

• Lee IC
• Kim BH
• Kim MK

• KMID: 1458935
• DOI: http://doi.org/10.5625/lar.2010.26.1.15

Abstract

In this study, we investigated the in vitro antioxidative effects, antimicrobial activities and single oral dose toxicity of the extracts from Samwhang-sasimtang to evaluate its use as a functional ingredient in cosmetics. In the antioxidative effect, the ethanol extract from Samwhang-sasimtang (SSE) had higher antioxidant values of 91.9% at 1,000 microgram/mL than that of water extract from Samwhang-sasimtang (SSW, 77.0%) when evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation decolorization of SSE was 82.2%, higher than that of the SSW (55.0%) and the antioxidant protection factors (APF) of SSW and SSE were 1.64 and 1.62 at 1,000 microgram/mL in concentration, respectively. This study was also undertaken to test the in vitro antimicrobial activity with the extracts of Samwhang-sasimtang. In general, the SSE showed the significant antimicrobial activity against Staphylococcus aureus and Staphylococcus epidermidis. In single oral dose toxicity study, there were no differences in vivo were observed between control and treated groups in clinical signs, body weight gains, and gross finding. The results indicated that SSE did not show any toxic effects at 10 mL/kg in mice, and the LD50 of SSE was found to be higher than 10 mL/kg in this experiment. In conclusion, the extracts from Samwhang-sasimtang may act as a natural subsistence for functional cosmetics.

Keyword

Samwhang-sasimtang; antioxidative activity; antimicrobial activity; single oral dose toxicity

MeSH Terms

Animals
Biphenyl Compounds
Body Weight
Cosmetics
Ethanol
Lethal Dose 50
Mice
Picrates
Staphylococcus aureus
Staphylococcus epidermidis
Water
Biphenyl Compounds
Cosmetics
Ethanol
Picrates
Water

Figure

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  Figure 1. Antioxidative effect of Samwhang-sasimtang extracts on DPPH radical scavenging activity. SSW, Samwhang-sasimtang water extracts; SSE, Samwhang-sasimtang ethanol extracts. Data shown are mean values with SD (n=3). The value of each group statistically significant as compared with control (∗P<0.05).

• 

  Figure 2. Antioxidative effect of Samwhang-sasimtang extracts measured by ABTS radical cation decolorization. SSW, Samwhang-sasimtang water extracts; SSE, Samwhang-sasimtang ethanol extracts. Data shown are mean values with SD (n=3). The value of each group statistically significant as compared with control (∗P<0.05).

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