Lab Anim Res.  2012 Dec;28(4):265-271. 10.5625/lar.2012.28.4.265.

Anti-obesity effects of Rapha diet(R) preparation in mice fed a high-fat diet

• Kim J
• Kyung J
• Kim D
• Choi EK
• Bang P
• Park D
• Kim YB

• KMID: 1431410
• DOI: http://doi.org/10.5625/lar.2012.28.4.265

Abstract

The anti-obesity activities of Rapha diet(R) preparation containing silkworm pupa peptide, Garcinia cambogia, white bean extract, mango extract, raspberry extract, cocoa extract, and green tea extract were investigated in mice with dietary obesity. Male C57BL/6 mice were fed a high-fat diet (HFD) containing 3% Rapha diet(R) preparation for 8 weeks, and blood and tissue parameters of obesity were analyzed. The HFD markedly enhanced body weight gain by increasing the weights of epididymal, perirenal, and mesenteric adipose tissues. The increased body weight gain induced by HFD was significantly reduced by feeding Rapha diet(R) preparation, in which decreases in the weight of abdominal adipose tissue and the size of abdominal adipocytes were confirmed by microscopic examination. Long-term feeding of HFD increased blood triglycerides and cholesterol levels, leading to hepatic lipid accumulation. However, Rapha diet(R) preparation not only reversed the blood lipid levels, but also attenuated hepatic steatosis. The results indicate that Rapha diet(R) preparation could improve HFD-induced obesity by reducing both lipid accumulation and the size of adipocytes.

Keyword

Obesity; high-fat diet; Rapha diet(R) preparation; lipid accumulation; adipocyte size

MeSH Terms

Abdominal Fat
Adipocytes
Animals
Body Weight
Bombyx
Cacao
Cholesterol
Diet, High-Fat
European Continental Ancestry Group
Garcinia cambogia
Humans
Male
Mangifera
Mice
Obesity
Pupa
Tea
Triglycerides
Weights and Measures
Cholesterol
Tea
Triglycerides

Figure

• 

  Figure 1 Daily feed consumption (g/mouse) by mice fed normal diet, high-fat diet (HFD) alone or containing 3% Rapha diet® preparation for 8 weeks.

• 

  Figure 2 Change in body weights of mice during feeding normal diet (circle), high-fat diet (HFD) alone (triangle) or containing 3% Rapha diet® preparation (square) for 8 weeks. *Significantly different from normal diet (P<0.05). #Significantly different from HFD alone (P<0.05).

• 

  Figure 3 Absolute (A) and relative (B) weights of epididymal, perirenal, and mesentaric fats from mice fed normal diet (white), high-fat diet (HFD) alone (black) or containing 3% Rapha diet® preparation (grey) for 8 weeks. *Significantly different from normal diet (P<0.05). #Significantly different from HFD alone (P<0.05).

• 

  Figure 4 A-C: Representative findings of perirenal adipose tissue of mice fed normal diet (A), high-fat diet (HFD) alone (B) or containing 3% Rapha diet® preparation (C) for 8 weeks. D: Epididymal, perirenal, and mesentaric adipocyte sizes of mice fed normal diet (white), HFD alone (black) or containing 3% Rapha diet® preparation (grey). *Significantly different from normal diet (P<0.05). #Significantly different from HFD alone (P<0.05). Bar=100 µm.

• 

  Figure 5 Representative findings of the liver of mice fed normal diet (A), high-fat diet alone (B) or containing 3% Rapha diet® preparation (C) for 8 weeks. Hematoxylin-eosin staining.

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