J Nutr Health.  2018 Jun;51(3):201-207. 10.4163/jnh.2018.51.3.201.

Anti-obesity and LDL-cholesterol lowering effects of silkworm hemolymph in C57BL/6N mice fed high fat diet

Affiliations
  • 1Department of Food and Nutrition, Chungnam National University, Daejeon 34134, Korea. sunly@cnu.ac.kr

Abstract

PURPOSE
Obesity, a worldwide epidemic, is associated with insulin resistance, hyperlipidemia, hypertension, cardiovascular disease, and certain cancers. Many strategies, including natural alternative anti-obesity agents, are used widely to prevent obesity. This study examined the effects of silkworm hemolymph on the weight control of C57BL/6N mice fed with a high-fat diet.
METHODS
The mice were divided into five groups: normal group (N), high-fat diet group (HFC), high-fat diet and silkworm hemolymph (at dose of 1 mL/kg BW (HFS-1), 5 mL/kg BW (HFS-5) and 10 mL/kg (HFS-10) for 12 weeks.
RESULTS
After 12 weeks treatment, the administration of silkworm hemolymph decreased the final body weight significantly along with a decrease in the weights of epididymal fat and total fat. The plasma LDL-cholesterol concentration was significantly lower in the HFS-1, HFS-5, and HFS-10 groups than in the HFC group. In addition, the leptin level of the HFS groups was significantly lower than those of the HFC group without a change in the plasma insulin concentration.
CONCLUSION
These findings suggest that the silkworm hemolymph may have the potential to prevent obesity.

Keyword

silkworm hemolymph; anti-obesity; LDL-cholesterol lowering effect

MeSH Terms

Animals
Anti-Obesity Agents
Body Weight
Bombyx*
Cardiovascular Diseases
Diet, High-Fat*
Hemolymph*
Hyperlipidemias
Hypertension
Insulin
Insulin Resistance
Leptin
Mice*
Obesity
Plasma
Weights and Measures
Anti-Obesity Agents
Insulin
Leptin

Reference

1. Woods SC, Seeley RJ, Porte D Jr, Schwartz MW. Signals that regulate food intake and energy homeostasis. Science. 1998; 280(5368):1378–1383.
Article
2. Weiser M, Frishman WH, Michaelson MD, Abdeen MA. The pharmacologic approach to the treatment of obesity. J Clin Pharmacol. 1997; 37(6):453–473.
Article
3. Surwit RS, Kuhn CM, Cochrane C, McCubbin JA, Feinglos MN. Diet-induced type II diabetes in C57BL/6J mice. Diabetes. 1988; 37(9):1163–1167.
Article
4. Antipatis VJ, Gill TP. Obesity as a global problem. In : Björntorp P, editor. International Textbook of Obesity. Chichester: John Wiley & Sons, Ltd.;2001. p. 3–22.
5. Asano N, Oseki K, Tomioka E, Kizu H, Matsui K. N-containing sugars from Morus alba and their glycosidase inhibitory activities. Carbohydr Res. 1994; 259(2):243–255.
6. Kimura T, Nakagawa K, Kubota H, Kojima Y, Goto Y, Yamagishi K, Oita S, Oikawa S, Miyazawa T. Food-grade mulberry powder enriched with 1-deoxynojirimycin suppresses the elevation of postprandial blood glucose in humans. J Agric Food Chem. 2007; 55(14):5869–5874.
Article
7. Thaipitakwong T, Numhom S, Aramwit P. Mulberry leaves and their potential effects against cardiometabolic risks: a review of chemical compositions, biological properties and clinical efficacy. Pharm Biol. 2018; 56(1):109–118.
Article
8. Miyahara C, Miyazawa M, Satoh S, Sakai A, Mizusaki S. Inhibitory effects of mulberry leaf extract on postprandial hyperglycemia in normal rats. J Nutr Sci Vitaminol (Tokyo). 2004; 50(3):161–164.
Article
9. Kim GN, Kwon YI, Jang HD. Mulberry leaf extract reduces postprandial hyperglycemia with few side effects by inhibiting α-glucosidase in normal rats. J Med Food. 2011; 14(7-8):712–717.
Article
10. Tsuduki T, Kikuchi I, Kimura T, Nakagawa K, Miyazawa T. Intake of mulberry 1-deoxynojirimycin prevents diet-induced obesity through increases in adiponectin in mice. Food Chem. 2013; 139(1-4):16–23.
Article
11. Ryu KS, Lee HS, Chung SH, Kang PD. An activity of lowering blood-glucose levels according to preparative conditions of silkworm powder. Korean J Sericult Sci. 1997; 39(1):79–85.
12. Han J, Inoue S, Isoda H. Effects of silkworm powder on glucose absorption by human intestinal epithelial cell line Caco-2. J Nat Med. 2007; 61(4):387–390.
Article
13. Jung EY, Lee HS, Lee HJ, Kim JM, Lee KW, Suh HJ. Feeding silk protein hydrolysates to C57BL/KsJ-db/db mice improves blood glucose and lipid profiles. Nutr Res. 2010; 30(11):783–790.
Article
14. Okazaki Y, Kakehi S, Xu Y, Tsujimoto K, Sasaki M, Ogawa H, Kato N. Consumption of sericin reduces serum lipids, ameliorates glucose tolerance and elevates serum adiponectin in rats fed a high-fat diet. Biosci Biotechnol Biochem. 2010; 74(8):1534–1538.
Article
15. Xue R, Wang Y, Cao G, Pan Z, Zheng X, Zhou W, Gong C. Lowering the blood glucose of diabetes mellitus mice by oral administration with transgenic human insulin-like growth factor I silkworms. J Agric Food Chem. 2012; 60(26):6559–6564.
Article
16. Chan EW, Lye PY, Wong SK. Phytochemistry, pharmacology, and clinical trials of Morus alba. Chin J Nat Med. 2016; 14(1):17–30.
17. Rhee WJ, Park TH. Silkworm hemolymph inhibits baculovirus-induced insect cell apoptosis. Biochem Biophys Res Commun. 2000; 271(1):186–190.
Article
18. Choi SS, Rhee WJ, Park TH. Inhibition of human cell apoptosis by silkworm hemolymph. Biotechnol Prog. 2002; 18(4):874–878.
Article
19. Choi SS, Rhee WJ, Kim EJ, Park TH. Enhancement of recombinant protein production in Chinese hamster ovary cells through anti-apoptosis engineering using 30Kc6 gene. Biotechnol Bioeng. 2006; 95(3):459–467.
20. Yu W, Ying H, Tong F, Zhang C, Quan Y, Zhang Y. Protective effect of the silkworm protein 30Kc6 on human vascular endothelial cells damaged by oxidized low density lipoprotein (Ox-LDL). PLoS One. 2013; 8(6):e68746.
Article
21. Koo TY, Park JH, Park HH, Park TH. Beneficial effect of 30Kc6 gene expression on production of recombinant interferon-b in serum-free suspension culture of CHO cells. Process Biochem. 2009; 44(2):146–153.
22. Thupari JN, Kim EK, Moran TH, Ronnett GV, Kuhajda FP. Chronic C75 treatment of diet-induced obese mice increases fat oxidation and reduces food intake to reduce adipose mass. Am J Physiol Endocrinol Metab. 2004; 287(1):E97–E104.
Article
23. Yun SN, Moon SJ, Ko SK, Im BO, Chung SH. Wild ginseng prevents the onset of high-fat diet induced hyperglycemia and obesity in ICR mice. Arch Pharm Res. 2004; 27(7):790–796.
Article
24. Miller WC, Lindeman AK, Wallace J, Niederpruem M. Diet composition, energy intake, and exercise in relation to body fat in men and women. Am J Clin Nutr. 1990; 52(3):426–430.
Article
25. Morton GJ, Gelling RW, Niswender KD, Morrison CD, Rhodes CJ, Schwartz MW. Leptin regulates insulin sensitivity via phosphatidylinositol-3-OH kinase signaling in mediobasal hypothalamic neurons. Cell Metab. 2005; 2(6):411–420.
Article
26. Sullivan PW, Ghushchyan VH, Ben-Joseph R. The impact of obesity on diabetes, hyperlipidemia and hypertension in the United States. Qual Life Res. 2008; 17(8):1063–1071.
Article
27. Yatsunami K, Ichida M, Onodera S. The relationship between 1-deoxynojirimycin content and alpha-glucosidase inhibitory activity in leaves of 276 mulberry cultivars (Morus spp.) in Kyoto, Japan. J Nat Med. 2008; 62(1):63–66.
28. Lafontan M, Girard J. Impact of visceral adipose tissue on liver metabolism. Part I: heterogeneity of adipose tissue and functional properties of visceral adipose tissue. Diabetes Metab. 2008; 34(4 Pt 1):317–327.
29. Brennan AM, Mantzoros CS. Drug Insight: the role of leptin in human physiology and pathophysiology--emerging clinical applications. Nat Clin Pract Endocrinol Metab. 2006; 2(6):318–327.
Article
30. Lin S, Thomas TC, Storlien LH, Huang XF. Development of high fat diet-induced obesity and leptin resistance in C57Bl/6J mice. Int J Obes Relat Metab Disord. 2000; 24(5):639–646.
Article
31. Scarpace PJ, Zhang Y. Leptin resistance: a prediposing factor for diet-induced obesity. Am J Physiol Regul Integr Comp Physiol. 2009; 296(3):R493–R500.
Article
32. Maffei M, Halaas J, Ravussin E, Pratley RE, Lee GH, Zhang Y, Fei H, Kim S, Lallone R, Ranganathan S, Kern PA, Friedman JM. Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nat Med. 1995; 1(11):1155–1161.
Article
33. Choi SS, Rhee WJ, Park TH. Beneficial effect of silkworm hemolymph on a CHO cell system: Inhibition of apoptosis and increase of EPO production. Biotechnol Bioeng. 2005; 91(7):793–800.
Article
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