Ewha Med J.  1995 Dec;18(4):301-307. 10.12771/emj.1995.18.4.301.

Genomic Organization of Human beta3 Subunit Gene of Voltage-Gated Calcium Channel

• Kim HL

• KMID: 2078824
• DOI: http://doi.org/10.12771/emj.1995.18.4.301

Abstract

Voltage-gated calcium channel (VGCC) is composed of at least four (alpha1, alpha2, beta, and delta)subunits. Among them beta subunit accelerates the kinetics of activation (channel opening) and nactivation (channel closure), and reguates the channel activity by phosphprylation through PKAand PKC that are activated by various signal transduction mechanisms. Until recently four isoforms of beta subunits (beta1, beta2, beta3, beta4) have been identified. Our recent data shows that VDCCbeta3 gene is expressed only in e nervous system and around the perinatal stage at high level.Alternative splicing was o observed at both 5'- and 3'- ends. To elucidate alternative splicingand cts-acting element of gene regulation of the beta3 subunit gene we isolated a 12.5kb-sizedgenomic clone encompassing beta3 subunit gene from human genomic library using the whole beta3subunit cDNA from NG108-l5 cell line as a probe. The genome was analyzed by Southem hybridization and sequencing. The beta3 subunit gene consists at least of 12 exons, and deduced amino acid sequence from the exons showed 98% similarity with that of rat gene. The beta3 subunitgene is not alternatively spliced at the middle of the gene, and has many possible phosphorylation sites,which may confer the regulatory role of the beta3 subunit gene.

Keyword

Calcium channel; Human genome

MeSH Terms

Amino Acid Sequence
Animals
Calcium Channels*
Calcium*
Cell Line
Clone Cells
DNA, Complementary
Exons
Genome
Genome, Human
Genomic Library
Humans*
Kinetics
Nervous System
Phosphorylation
Protein Isoforms
Rats
Signal Transduction
Calcium
Calcium Channels
DNA, Complementary
Protein Isoforms