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Rapid detection of deformed wing virus in honeybee using ultra-rapid qPCR and a DNA-chip

Kim JM, Lim SJ, Kim S, Kim M, Kim B, Tai TA, Kim S, Yoon B

Fast and accurate detection of viral RNA pathogens is important in apiculture. A polymerase chain reaction (PCR)-based detection method has been developed, which is simple, specific, and sensitive. In this...
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CRTC2 as a novel prognostic biomarker for worse pathologic outcomes and biochemical recurrence after radical prostatectomy in patients with prostate cancer

Lee H, Lee M, Hong SK

PURPOSE: To identify the association between tumor metabolism and prostate cancer (PCa), we investigated the relationship between expression of metabolism-related genes and clinicopathologic outcomes in patients with localized PCa. MATERIALS AND...
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Ethanol changes atpB gene expression and proton permeability in Streptococcus mutans

Cho CM, Park YJ, Lee SA, Kim JB, Kang JS

OBJECTIVES: As a first step to study the anticaries effect of ethanol alone, we investigated the effects of ethanol on the expression levels of the atpB gene and proton permeability...
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Functional Identification of Compound Heterozygous Mutations in the CYP17A1 Gene Resulting in Combined 17α-Hydroxylase/17,20-Lyase Deficiency

Mo EY, Lee JY, Kim SY, Kim MJ, Kim ES, Lee S, Han JH, Moon SD

BACKGROUND: We previously reported a patient with congenital adrenal hyperplasia (CAH) with compound heterozygous mutations in the cytochrome P450 17A1 (CYP17A1) gene. One allele had a p.His373Leu and the other...
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Structural analyses of putative porcine cluster of differentiation (CD) 24 gene

Ko H, Chai HH, Jang GW, Park JE, Kim JM, Lee KT

Cluster of differentiation (CD) 24 or heat stable antigen 24 (HSA) molecule is a mucin-type glycoprotein attached to the cell surface by glycosylphosphatidylinositol (GPI)-anchor, promoting adhesive interactions between cells or...
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Characterization of Echinostoma cinetorchis endoribonuclease, RNase H

Lim SB, Cha SH, Jegal S, Jun H, Park SH, Jeon BY, Pak JH, Bakh YY, Kim TS, Lee HW

Echinostoma cinetorchis is an oriental intestinal fluke causing significant pathological damage to the small intestine. The aim of this study was to determine a full-length cDNA sequence of E. cinetorchis...
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Identification and Characterization of Protein Arginine Methyltransferase 1 in Acanthamoeba castellanii

Moon EK, Kong HH, Hong Y, Lee HA, Quan FS

Protein arginine methyltransferase (PRMT) is an important epigenetic regulator in eukaryotic cells. During encystation, an essential process for Acanthamoeba survival, the expression of a lot of genes involved in the...
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Detection of Methylated Circulating DNA as Noninvasive Biomarkers for Breast Cancer Diagnosis

Cheuk IW, Shin VY, Kwong A

Internationally, breast cancer is the most common female cancer, and is induced by a combination of environmental, genetic, and epigenetic risk factors. Despite the advancement of imaging techniques, invasive sampling...
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Single fecal microbiota transplantation failed to change intestinal microbiota and had limited effectiveness against ulcerative colitis in Japanese patients

Mizuno S, Nanki K, Matsuoka K, Saigusa K, Ono K, Arai M, Sugimoto S, Kiyohara H, Nakashima , Takeshita , Naganuma M, Suda W, Hattori M, Kanai T

BACKGROUND/AIMS: Recent developments in analytical techniques including next-generation sequencing have clarified the correlation between intestinal microbiota and inflammatory bowel disease. Fecal microbiota transplantation (FMT) for patients with ulcerative colitis (UC)...
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Molecular Cloning and Characterization of a P38-Like Mitogen-Activated Protein Kinase from Echinococcus granulosus

Lü G, Li J, Zhang C, Li L, Bi X, Li C, Fan J, Lu X, Vuitton DA, Wen H, Lin R

Cystic echinococcosis (CE) treatment urgently requires a novel drug. The p38 mitogen-activated protein kinases (MAPKs) are a family of Ser/Thr protein kinases, but still have to be characterized in Echinococcus...
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Role of Rotavirus Enterotoxin NSP4 in the Inflammatory Response in Murine Macrophage RAW 264.7 Cells

Kim SA, Than VT, Kim W

The rotavirus nonstructural glycoprotein, NSP4, has been identified as the first viral enterotoxin capable of inducing diarrhea. To investigate the biological function of NSP4 in the inflammatory process, a cDNA...
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Viral Effects of a dsRNA Mycovirus (PoV-ASI2792) on the Vegetative Growth of the Edible Mushroom Pleurotus ostreatus

Song HY, Choi HJ, Jeong H, Choi D, Kim DH, Kim JM

A double-stranded RNA (dsRNA) mycovirus was detected in malformed fruiting bodies of Pleurotus ostreatus strain ASI2792, one of bottle cultivated commercial strains of the edible oyster mushroom. The partial RNA-dependent...
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Metallothinein 1E Enhances Glioma Invasion through Modulation Matrix Metalloproteinases-2 and 9 in U87MG Mouse Brain Tumor Model

Hur H, Ryu HH, Li CH, Kim IY, Jang WY, Jung S

Malignant glioma cells invading surrounding normal brain are inoperable and resistant to radio- and chemotherapy, and eventually lead to tumor regrowth. Identification of genes related to motility is important for...
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Vacuolar Serine Protease Is a Major Allergen of Fusarium proliferatum and an IgE-Cross Reactive Pan-Fungal Allergen

Yeh CC, Tai HY, Chou H, Wu KG, Shen HD

PURPOSE: Fusarium species are among prevalent airborne fungi and causative agents of human respiratory atopic disorders. We previously identified a 36.5-kDa F. proliferatum component recognized by IgE antibodies in 9...
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In situ Hybridization for the Detection and Localization of the Bitter Taste Receptor Tas2r108 in the Murine Submandibular Gland

Ki SY, Cho YK, Chung KM, Kim KN

Mammals have 3 pairs of major salivary glands i.e., the parotid, submandibular, and sublingual glands. Saliva secretion of these glands is modulated by taste perception. Salivary glands are composed mainly...
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Aberrant Expression of Breast Development-Related MicroRNAs, miR-22, miR-132, and miR-212, in Breast Tumor Tissues

Damavandi , Torkashvand S, Vasei M, Soltani BM, Tavallaei , Mowla SJ

PURPOSE: MicroRNAs (miRNAs) are a major class of small endogenous RNA molecules that posttranscriptionally regulate the expression of most genes in the human genome. miRNAs are often located in chromosomal...
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RNA sequencing of the nephron transcriptome: a technical note

Lee JW

To understand the functions of the kidney, the transcriptome of each part of the nephron needs to be profiled using a highly sensitive and unbiased tool. RNA sequencing (RNA-seq) has...
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Phylogeography of the current rabies viruses in Indonesia

Dibia IN, Sumiarto B, Susetya H, Putra AA, Scott-Orr , Mahardika

Rabies is a major fatal zoonotic disease in Indonesia. This study was conducted to determine the recent dynamics of rabies virus (RABV) in various areas and animal species throughout Indonesia....
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Establishment and evaluation of a murine alphavbeta3-integrin-expressing cell line with increased susceptibility to Foot-and-mouth disease virus

Zhang W, Lian K, Yang F, Yang Y, Zhu Z, Zhu Z, Cao W, Mao R, Jin Y, He J, Guo J, Liu X, Zheng H

Integrin alphavbeta3 plays a major role in various signaling pathways, cell apoptosis, and tumor angiogenesis. To examine the functions and roles of alphavbeta3 integrin, a stable CHO-677 cell line expressing...
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Distribution of 5-HT3, 5-HT4, and 5-HT7 Receptors Along the Human Colon

Yaakob NS, Chinkwo KA, Chetty N, Coupar IM, Irving HR

  • KMID: 2433475
  • J Neurogastroenterol Motil.
  • 2015 Jul;21(3):361-369.
  • doi: 10.5056/jnm14157
BACKGROUND/AIMS: Several disorders of the gastrointestinal tract are associated with abnormal serotonin (5-HT) signaling or metabolism where the 5-HT3 and 5-HT4 receptors are clinically relevant. The aim was to examine...
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