Korean J Psychopharmacol.  2001 Jun;12(2):140-146.

The Role of 5-HT Receptors on the Acetylcholine Release from the Rat Striatum

Affiliations
  • 1Department of Pharmacology, School of Medicine, Wonkwang University, Iksan, Korea. cbk@wonkwang.ac.kr

Abstract

The aim of this study was to investigate the role of the 5-HT receptors in acetylcholine (ACh) release from the striatum. Slices from the rat striatum and synaptosomes were incubated with [3H]-choline and the release of the labelled products was evoked by electrical (3 Hz, 2 ms, 5 V/cm, rectangular pulses, 2 min) and potassium-stimulation (25 mM), respectively, and the influence of various serotonergic drugs on the evoked tritium outflows was investigated. Serotonin decreased the electrically-evoked ACh release in striatum in a concentration-dependent manner without the change of basal release. In hippocampal and entorhinal cortical slices, serotonin did not affect the evoked and basal release of ACh, but, at large dose (30 microM) decreased the evoked ACh release in hippocampus. 2,5-Dimethoxy-4-iodoamphetamine (DOI), a specific 5-HT 2A/2C agonist, decreased evoked ACh release in the striatum. CGS-12066A (5-HT 1B agonist), m-chlorophenyl-biguanide (5-HT 3 agonist) and 5-[(dimethyl -amino)methyl]-3-(1-methyl-1H-indol-3-yl)-1,2,4-oxadiazole (5-HT 3 antagonist) did not affect the evoked and basal ACh release in all tissues. Ritanserin, a specific 5-HT 2A/2C antagonist, blocked the inhibitory effects of serotonin and DOI, whereas, ketanserin, an another type of specific 5-HT 2A/2C antagonist did not affect the inhibitory effects of serotonin and DOI. In striatal synaptosomal preparation, serotonin and DOI did not affect the K +-evoked ACh release. These findings suggest that ritanserin-sensitive 5-HT 2A/2C receptors located in the soma and/or axons of the striatal cholinergic neurons play a important role in ACh release.

Keyword

Serotonin; Acetylcholine; Striatum; 5-HT receptors; Rat

MeSH Terms

Acetylcholine*
Animals
Axons
Carisoprodol
Cholinergic Neurons
Hippocampus
Ketanserin
Rats*
Receptors, Serotonin*
Ritanserin
Serotonin Agents
Serotonin*
Synaptosomes
Tritium
Acetylcholine
Carisoprodol
Ketanserin
Receptors, Serotonin
Ritanserin
Serotonin
Serotonin Agents
Tritium
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