Korean J Physiol Pharmacol.  2004 Dec;8(6):301-305.

Effects of NMDA, AMPA and Kainate on the Release of Acetylcholine in Rat Hippocampal and Striatal Slices

  • 1Department of Pharmacology, Wonkwang University School of Medicine, Iksan 570-749, Korea. cbk@wonkwang.ac.kr
  • 2Department of Oral Physiology, Chosun University College of Dentistry, Gwangju 501-759, Korea.
  • 3Department of Pharmacology, Chonnam National University Medical School, Chonnam National University Research Institute of Medical Sciences, Gwangju 501-746, Korea.


This study examined the effects of N-methyl-D-aspartate (NMDA), alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) and kainate on basal and electrically-evoked release of acetylcholine (ACh) from the rat hippocampal and striatal slices which were preincubated with [3H]choline. Unexpectedly, the basal and evoked ACh release were not affected at all by the treatment with NMDA (3~100microM), AMPA (1~100microM) or kainate (1~100microM) in hippocampal slices. However, in striatal slices, under the Mg2 -free medium, 30microM NMDA increased the basal ACh release with significant decrease of the electrically- evoked releases. The treatment with 1microM MK-801 not only reversed the 30microM NMDA-induced decrease of the evoked ACh release, but also attenuated the facilitatory effect of 30microM NMDA on the basal ACh release. The treatment with either 30microM AMPA or 100microM kainate increased the basal ACh release without any effects on the evoked release. The treatment with 10microM NBQX abolished the AMPA- or kainate-induced increase of the basal ACh release. Interestingly, NBQX significantly attenuated the evoked release when it was treated with AMPA, although it did not affect the evoked release alone without AMPA. These observations demonstrate that in hippocampal slices, ionotropic glutamate receptors do not modulate the ACh release in cholinergic terminals, whereas in striatal slices, activations of ionotropic glutamate receptors increase the basal ACh release though NMDA may decrease the electrically-evoked ACh release.


NMDA; AMPA; Kainate; Acetylcholine release; Hippocampus; Striatum

MeSH Terms

alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid*
Dizocilpine Maleate
Kainic Acid*
Receptors, Ionotropic Glutamate
Dizocilpine Maleate
Kainic Acid
Receptors, Ionotropic Glutamate
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
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