Anat Cell Biol.  2011 Jun;44(2):106-115. 10.5115/acb.2011.44.2.106.

Immunohistochemical study on the expression of calcium binding proteins (calbindin-D28k, calretinin, and parvalbumin) in the cerebral cortex and in the hippocampal region of nNOS knock-out(-/-) mice

Affiliations
  • 1Department of Anatomy, College of Medicine, Seoul National University, Seoul, Korea. kmjoo@snu.ac.kr
  • 2Department of Neurosurgery, School of Medicine, Sungkyunkwan University, Seoul, Korea.

Abstract

Nitric oxide (NO) modulates the activities of various channels and receptors to participate in the regulation of neuronal intracellular Ca2+ levels. Ca2+ binding protein (CaBP) expression may also be altered by NO. Accordingly, we examined expression changes in calbindin-D28k, calretinin, and parvalbumin in the cerebral cortex and hippocampal region of neuronal NO synthase knockout(-/-) (nNOS-/-) mice using immunohistochemistry. For the first time, we demonstrate that the expression of CaBPs is specifically altered in the cerebral cortex and hippocampal region of nNOS-/- mice and that their expression changed according to neuronal type. As changes in CaBP expression can influence temporal and spatial intracellular Ca2+ levels, it appears that NO may be involved in various functions, such as modulating neuronal Ca2+ homeostasis, regulating synaptic transmission, and neuroprotection, by influencing the expression of CaBPs. Therefore, these results suggest another mechanism by which NO participates in the regulation of neuronal Ca2+ homeostasis. However, the exact mechanisms of this regulation and its functional significance require further investigation.

Keyword

Calcium binding proteins; Cerebral cortex and hippocampal region; Immunohistochemistry; Neuronal nitric oxide synthase (nNOS); nNOS knock-out(-/-) mice
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