Lab Anim Res.  2018 Dec;34(4):270-278. 10.5625/lar.2018.34.4.270.

Comparison of the anesthetic effects of 2,2,2-tribromoethanol on ICR mice derived from three different sources

• Lee MR
• Suh HR
• Kim MW
• Cho JY
• Song HK
• Jung YS
• Hwang DY
• Kim KS

• KMID: 2459305
• DOI: http://doi.org/10.5625/lar.2018.34.4.270

Abstract

This study was conducted to compare the anesthetic effects of 2,2,2-tribromoethanol (TBE, Avertin®) in ICR mice obtained from three different sources. TBE (2.5%) was intraperitoneally injected at three doses: high-dose group (500 mg/kg), intermediate-dose group (250 mg/kg), and low-dose group (125 mg/kg). Anesthesia time, recovery time, end-tidal peak CO2 (ETCOâ‚‚), mean arterial blood pressure, heart rate, oxygen saturation (SpOâ‚‚), body temperature, pH, PCOâ‚‚, and POâ‚‚ of the arterial blood were measured. Stable anesthesia was induced by all doses of TBE and the anesthesia time was maintained exhibited dose dependency. No significant differences in anesthetic duration were found among the three different strains. However, the anesthesia time was longer in female than in male mice, and the duration of anesthesia was significantly longer in female than in male mice in the high-dose group. The recovery time was significantly longer for female than male mice in the intermediate- and high-dose groups. In the ICR strains tested, there were no significant differences in the mean arterial blood pressure, SPOâ‚‚, arterial blood PCOâ‚‚, and POâ‚‚, which decreased after TBE anesthesia, or in heart rate and ETCOâ‚‚, which increased after TBE anesthesia. In addition, body temperature, blood biochemical markers, and histopathological changes of the liver, kidney, and lung were not significantly changed by TBE anesthesia. These results suggested that ICR mice from different sources exhibited similar overall responses to a single exposure to TBE anesthesia. In conclusion, TBE is a useful drug that can induce similar anesthetic effects in three different strains of ICR mice.

Keyword

Anesthesia effect; Korl:ICR mice; pulse oximeter; sex difference; tribromoethanol

MeSH Terms

Anesthesia
Anesthetics*
Animals
Arterial Pressure
Biomarkers
Body Temperature
Female
Heart Rate
Humans
Hydrogen-Ion Concentration
Kidney
Liver
Lung
Male
Mice
Mice, Inbred ICR*
Oxygen
Sex Characteristics
Anesthetics
Biomarkers
Oxygen

Figure

• 

  Figure 1 Anesthesia time and recovery time in male and female ICR mice administered the TBE. The data shown represent the means±SD (n=7 per group). A P value less than 0.05 was considered to be statistically significant. *P<0.05 significant difference vs. male Korl:ICR group. #P<0.05 significant difference vs. male A:ICR group. §P<0.05 significant difference vs. male B:ICR group.

• 

  Figure 2 Mean arterial blood pressure, heart rate, end-tidal peak CO2, and SpO2 in male and female ICR mice administered the TBE. Data are presented as means±SD. A P value less than 0.05 was considered to be statistically significant. There were no significant differences between dose, sex, and ICR strain.

• 

  Figure 3 Arterial blood PCO2, and PO2 in male and female ICR mice administered the TBE. Data are presented as means±SD. A P value less than 0.05 was considered to be statistically significant. There were no significant differences between dose, sex, and ICR strain.

• 

  Figure 4 The histological appearances of liver, kidney, and lung in male and female ICR mice administered the TBE. The section were stained using hematoxylin and eosin. Magnification ×200.

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