Lab Anim Res.  2017 Jun;33(2):140-149. 10.5625/lar.2017.33.2.140.

Comparative analysis of basal locomotor activity-related metabolic phenotypes between C57BL/6 mice and ICR mice substrains derived from three different sources

Affiliations
  • 1Exercise Biochemistry Laboratory, Korea National Sport University, 88-15 Oryun-dong, Songpa-gu, Seoul 138-763, Korea. chojy86@knsu.ac.kr
  • 2Department of Microbiology and Immunology, INJE University College of Medicine, Busan 47392, Korea.
  • 3College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea.
  • 4College of Pharmacy, Pusan National University, Busan 46241, Korea.
  • 5Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Korea.

Abstract

Animal model, as an indispensable tool for scientific purposes of biomedical researches and clinical application, is a commonly used in various researches. Regarding to this, it is necessary to establish the metabolic phenotype of animal model to minimize spurious interpretations and ensure a level of accuracy and reliability adequate for experimental research. However, the metabolic phenotype-related analysis within rodent strains derived from different source is nonexistent, especially in C57BL/6Korl mice and Korl:ICR mice (a domestic mouse strain). To compare the physiological and metabolic phenotypes over a period of time, we utilized the C57BL/6 mice (C57BL/6Korl, A:C57BL/6, and B:C57BL/6) and ICR mice (Korl:ICR, A:ICR, and B:ICR) derived from three different sources. Our data showed that average body weight, body temperature, food intake, and water consumption have a similar tendency among the C57BL/6 and ICR groups, except for the higher body weight of C57BL/6Korl mice over a period of time. Moreover, some significant differences was observed in adipose tissue mass and adipocyte size among the groups, with a higher tendency of C57BL/6Korl mice and Korl:ICR mice. Most importantly, resting metabolic rate (RMR) serves as an approximation of the metabolic phenotype showed no significant difference among the groups of C57BL/6 mice and ICR mice, except for the lower oxygen uptake of C57BL/6Korl mice compare to the A:C57BL/6 mice. Taken together, our data suggest that C57BL/6 mice and ICR mice derived from three different sources have an overall similar feature of physiological and metabolic phenotypes.

Keyword

C57BL/6Korl; Korl:ICR; metabolic phenotype; resting metabolic rates

MeSH Terms

Adipocytes
Adipose Tissue
Animals
Body Temperature
Body Weight
Drinking
Eating
Mice*
Mice, Inbred ICR*
Models, Animal
Oxygen
Phenotype*
Rodentia
Oxygen

Figure

  • Figure 1 Comparative analysis of the physiological features of three C57BL/6 subgroups and three ICR subgroups. (A) Body weight changes over a period of time in C57BL/6 mice. (B, C) Average body weight and body temperature of C57BL/6 mice. (D, E) Changes in food intake and water consumption over a period of time in C57BL/6 mice. F) Body weight changes over a period of time in ICR mice. (G, H) Average body weight and body temperature in ICR mice. (I, J) Changes in food intake and water consumption over a period of time in ICR mice. Values are presented as mean±SD (5 mice per subgroup); *P<0.05 versus the C57BL/6Korl subgroup.

  • Figure 2 Comparative analysis of adipose tissue mass and adipocyte size among three C57BL/6 subgroups. (A) Epididymal adipose tissue mass. B) Retroperitoneal adipose tissue mass. (C, D) Histological analysis of epididymal and retroperitoneal adipose tissue. (E) Epididymal adipocyte size. (F) Retroperitoneal adipocyte size. Scale bar: 200 µm. Values for each subgroup are presented as mean±SD from 5 mice.

  • Figure 3 Comparative analysis of adipose tissue mass and adipocyte size among three ICR subgroups. (A) Epididymal adipose tissue mass. B) Retroperitoneal adipose tissue mass. (C, D) Histological analysis of epididymal and retroperitoneal adipose tissue. (E) Epididymal adipocyte size. (F) Retroperitoneal adipocyte size. Scale bar: 200 µm. Values for each subgroup are presented as mean±SD from 5 mice.

  • Figure 4 Comparative analysis of the metabolic phenotypes of three C57BL/6 subgroups during a period of 24 hours at RMR. (A) Changes of oxygen uptake (left) and total oxygen uptake (right), (B) Changes in CO2 production (left) and total oxygen uptake (right), (C) Changes of RER value (left) and average RER (right), (D) Changes of carbohydrate oxidation (left) and total carbohydrate oxidation (right), and (E) Changes of fat oxidation (left) and total fat oxidation (right). Values are presented as mean±SD; each subgroup consisted of 4-5 mice.

  • Figure 5 Comparative analysis of the metabolic phenotypes of three ICR subgroups during a period of 24 hours at RMR. (A) Changes of oxygen uptake (left) and total oxygen uptake (right), (B) Changes in CO2 production (left) and total oxygen uptake (right), (C) Changes of RER value (left) and average RER (right), (D) Changes of carbohydrate oxidation (left) and total carbohydrate oxidation (right), and (E) Changes of fat oxidation (left) and total fat oxidation (right). Values are presented as mean±SD; each subgroup consisted of 4-5 mice.


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