Ann Lab Med.  2017 Sep;37(5):398-407. 10.3343/alm.2017.37.5.398.

Multidrug Resistance Mechanisms of Carbapenem Resistant Klebsiella pneumoniae Strains Isolated in Chongqing, China

Affiliations
  • 1Department of Clinical Laboratory, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. 1309898173@qq.com

Abstract

BACKGROUND
Carbapenem-resistant Klebsiella pneumoniae (CRKP) is considered a serious global threat. However, little is known regarding the multidrug resistance (MDR) mechanisms of CRKP. This study investigated the phenotypes and MDR mechanisms of CRKP and identified their clonal characteristics.
METHODS
PCR and sequencing were utilized to identify antibiotic resistance determinants. Integron gene cassette arrays were determined by restriction fragment length polymorphism (RFLP) analysis. Multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were used for epidemiological analysis. Plasmids were typed by using a PCR-based replicon typing and analyzed by conjugation and transformation assays.
RESULTS
Seventy-eight strains were identified as resistant to at least one carbapenem; these CRKP strains had a high prevalence rate (38.5%, 30/78) of carbapenemase producers. Additionally, most isolates harbored MDR genes, including Extended spectrum β-lactamases (ESBLs), AmpC, and quinolone and aminoglycoside resistance genes. Loss of porin genes was observed, and Class 1 integron was detected in 66.7% of the investigated isolates. PFGE and MLST results excluded the occurrence of clonal dissemination among these isolates.
CONCLUSIONS
A high prevalence of NDM-1 genes encoding carbapenem resistance determinants was demonstrated among the K. pneumoniae isolates. Importantly, this is the first report of bla(NDM-1) carriage in a K. pneumoniae ST1383 clone in China and of a MDR CRKP isolate co-harboring bla(NDM-1), bla(KPC-2), bla(CTX-M), bla(SHV), acc(6"²)-Ib, rmtB, qnrB, and acc(6"²)-Ib-cr.

Keyword

Carbapenem-resistant; Klebsiella pneumoniae; Carbapenemases; Extended spectrum β-lactamases; Molecular epidemiology

MeSH Terms

China*
Clone Cells
Drug Resistance, Bacterial
Drug Resistance, Microbial
Drug Resistance, Multiple*
Electrophoresis, Gel, Pulsed-Field
Genes, MDR
Integrons
Klebsiella pneumoniae*
Klebsiella*
Molecular Epidemiology
Phenotype
Plasmids
Pneumonia
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Prevalence
Replicon
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