Ann Lab Med.  2018 Jul;38(4):367-370. 10.3343/alm.2018.38.4.367.

Phenotypic and Genomic Characterization of AmpC-Producing Klebsiella pneumoniae From Korea

• Palmieri M
• Schicklin
• Pelegrin A
• Chatellier S
• Franceschi C
• Mirande C
• Park YJ
• van Belkum A

• KMID: 2408077
• DOI: http://doi.org/10.3343/alm.2018.38.4.367

Abstract

The prevalence of multidrug-resistant gram-negative bacteria has continuously increased over the past few years; bacterial strains producing AmpC β-lactamases and/or extended-spectrum β-lactamases (ESBLs) are of particular concern. We combined high-resolution whole genome sequencing and phenotypic data to elucidate the mechanisms of resistance to cephamycin and β-lactamase in Korean Klebsiella pneumoniae strains, in which no AmpC-encoding genes were detected by PCR. We identified several genes that alone or in combination can potentially explain the resistance phenotype. We showed that different mechanisms could explain the resistance phenotype, emphasizing the limitations of the PCR and the importance of distinguishing closely-related gene variants.

Keyword

AmpC; Antibiotic resistance; Whole Genome Sequencing; Klebsiella pneumoniae; Korea

MeSH Terms

Drug Resistance, Microbial
Genome
Gram-Negative Bacteria
Klebsiella pneumoniae*
Klebsiella*
Korea*
Phenotype
Polymerase Chain Reaction
Prevalence

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