Toxicol Res.
2013 Mar;29(1):61-67.
Protective Effects of Houttuynia cordata Thunb. on Gentamicin-induced Oxidative Stress and Nephrotoxicity in Rats
- Affiliations
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- 1Department of Pharmacology & Toxicology, College of Veterinary Medicine, Gyeongsang National University, Jinju, Korea. jskim@gnu.ac.kr
- 2Gyeongnam Livestock promotion Research Institute, Jinju, Korea.
- 3Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology, Jinju, Korea.
Abstract
- Development of a therapy providing protection from, or reversing gentamicin-sulfate (GS)-induced oxidative stress and nephrotoxicity would be of great clinical significance. The present study was designed to investigate the protective effects of Houttuynia cordata Thunb. (HC) against gentamicin sulfate-induced renal damage in rats. Twenty-eight Sprague-Dawley rats were divided into 4 equal groups as follows: group 1, control; group 2, GS 100 mg/kg/d, intraperitoneal (i.p.) injection; group 3, GS 100 mg/kg/d, i.p. + HC 500 mg/kg/d, oral; and group 4, GS 100 mg/kg/d i.p. + HC 1000 mg/kg/d, oral administration). Treatments were administered once daily for 12 d. After 12 d, biochemical and histopathological analyses were conducted to evaluate oxidative stress and renal nephrotoxicity. Serum levels of creatinine, malondialdehyde (MDA), and blood urea nitrogen (BUN), together with renal levels of MDA, glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) were quantified to evaluate antioxidant activity. Animals treated with GS alone showed a significant increase in serum levels of creatinine, BUN, and MDA, with decreased renal levels of GSH, SOD, and CAT. Treatment of rats with HC showed significant improvement in renal function, presumably as a result of decreased biochemical indices and oxidative stress parameters associated with GS-induced nephrotoxicity. Histopathological examination of the rat kidneys confirmed these observations. Therefore, the novel natural antioxidant HC may protect against GS-induced nephrotoxicity and oxidative stress in rats.
Keyword
MeSH Terms
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Animals
Blood Urea Nitrogen
Catalase
Cats
Creatinine
Drug Combinations
Gentamicins
Glutathione
Glycerides
Houttuynia
Kidney
Malondialdehyde
Monoterpenes
Oxidative Stress
Rats
Rats, Sprague-Dawley
Superoxide Dismutase
Catalase
Creatinine
Drug Combinations
Gentamicins
Glutathione
Glycerides
Malondialdehyde
Monoterpenes
Superoxide Dismutase
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