Yonsei Med J.  1988 Sep;29(3):225-232. 10.3349/ymj.1988.29.3.225.

Protective Effect of Urinary Alkalinization on Gentamicin Nephrotoxicity in Rats

• Lee HY
• Choi KH
• Bank N

• KMID: 1729551
• DOI: http://doi.org/10.3349/ymj.1988.29.3.225

Abstract

We investigated the effect of urinary alkalinization accomplished by intraperitoneal injection of sodium bicarbonate and acetazolamide on gentamicin nephrotoxicity in male Fisher 344rats. Forty rats (body weight 200-300g) were divided into four groups: control (gentamicin 20mg/kg, bid), high sodium load (gentamicin 20mg/kg, 25cc of saline, bid), low bicarbonate (gentamicin 20mg/kg, 25cc of sodium bicarbonate 100mEq/L, 2.5mg of Diamox, bid) and high bicarbonate (gentamicin 20mg/kg, 10cc of sodium bicarbonate 250mEq/L, 2.5mg of Diamox, bid) groups. All drugs and electrolyte solutions as mentioned above were administered intraperitoneally twice a day for seven days and changes in renal functions were studied. While salt loading failed to influence the severity of gentamicin nephrotoxicity, urinary alkalinization induced by bicarbonate and acetazolamide injection showed remarkable ameliorating effects on gentamicin nephrotoxicity. The high bicarbonate group exhibited more beneficial effects than the low bicarbonate group on gentamicin nephrotoxicity. So, urinary alkalinization seems to be an effective method for the prevention of gentamicin nephrotoxicity in rats.

Keyword

Urinary alkalinization; gentamicin nephrotoxicity

MeSH Terms

Animal
Bicarbonates/*administration and dosage
Carbon Dioxide/urine
Gentamicins/*toxicity
Kidney Diseases/chemically induced/*prevention and control
Male
Random Allocation
Rats
Rats, Inbred F344