Korean J Biol Psychiatry.
2007 May;14(2):115-121.
Microarray Analysis of Gene Expression in Rat Glioma after Ethanol Treatment
- Affiliations
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- 1Department of Psychiatry, National Medical Center, Seoul, Korea.
- 2Department of Psychiatry, Myongji Hospital, College of Medicine, Kwandong University, Goyang, Korea. dongyulo@chollian.net
- 3Department Neuropsychiatry, St. Vincent Hospital, College of Medicine, The Catholic University of Korea, Suwon, Korea.
- 4Department of Psychiatry, Han-Gang Sacred Heart Hospital, College of Medicine, Hallym University, Seoul, Korea.
- 5Department Neuropsychiatry, Our Lady of Mercy Hospital, College of Me-dicine, The Catholic University of Korea, Incheon, Korea.
- 6Seoul National Hospital, Seoul, Korea.
- 7Department of Biochemistry and Molecular Biology, Hanyang University College of Sciences, Ansan, Korea.
Abstract
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Objetives: Identification of target genes for ethanol in neurons is important for understanding its molecular and cellular mechanism of action and the neuropathological changes seen in alcoholics. The purpose of this study is to identify of altered gene expression after acute treatmet of ethanol in rat gliom cells.
METHODS
We used high density cDNA microarray chip to measure the expression patterns of multiple genes in cultured rat glioma cells. DNA microarrays allow for the simultaneous measurement of the expression of several hundreds of genes.
RESULTS
After comparing hybridized signals between control and ethanol treated groups, we found that treatment with ethanol increased the expression of 15 genes and decreased the expression of 12 genes. Upregulated genes included Orthodenticle(Drosophila) homolog 1, procollagen type II, adenosine A2a receptor, GATA-bindning protein 2. Downregulated genes included diacylglycerol kinase beta, PRKC, Protein phosphatase 1, clathrin-associated protein 17, nucleoporin p58, proteasome.
CONCLUSION
The gene changes noted were those related to the regulation of transcription, signal transduction, second messenger systems. modulation of ischemic brain injury, and neurodengeneration.Although some of the genes were previously known to be ethanol responsive, we have for the most part identified novel genes involved in the brain response to ethanol.
Keyword
MeSH Terms
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Alcoholics
Animals
Brain
Brain Injuries
Collagen Type II
Diacylglycerol Kinase
Ethanol*
Gene Expression*
Glioma*
Humans
Microarray Analysis*
Neurons
Nuclear Pore Complex Proteins
Oligonucleotide Array Sequence Analysis
Proteasome Endopeptidase Complex
Protein Phosphatase 1
Rats*
Receptor, Adenosine A2A
Second Messenger Systems
Signal Transduction
Collagen Type II
Diacylglycerol Kinase
Ethanol
Nuclear Pore Complex Proteins
Proteasome Endopeptidase Complex
Protein Phosphatase 1
Receptor, Adenosine A2A
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