J Korean Cancer Assoc.  1997 Apr;29(2):266-279.

Prognostic Significance of Histologic Features, DNA Content, Expression of Proliferating Cell Nuclear Antigen (PCNA), c-fos Protein and Transforming Growth Factor (TGF)-alpha and -beta in Giant Cell Tumor of Bone

Affiliations
  • 1Department of Pathology, Kosin University Medical Center, Korea.
  • 2Department of Orthopedic Surgery, Kosin University Medical Center, Korea.

Abstract

PURPOSE: This study was attempted to investigate the prevalence of the expression of c-fos protein, TGF-alpha and -beta, PCNA , DNA ploidy pattern and histopathological parameters of giant cell tumor (GCT) of bone and to correlate with prognosis and to extend our understanding on tumorigenesis of GCT.
MATERIALS AND METHODS
Twenty eight cases of paraffin-embedded tissue were studied, classified as recurrent (5 cases) and non-recurrent group (12cases) within the limits of the cases which afforded surgical material on first operation.
RESULTS
No significant difference was observed in cellularity of stromal cells, atypia of stromal and giant cells, presence of hemorrhage and necrosis between recurrent and non-recurrent group. However, presence of more than 10 mitotic figures in 10 high power fields in recurrent group was significantly higher than non-recurrent group (p<0.05). The immunoreactivity for PCNA was seen only in nuclei of stromal cells, whereas nuclei of giant cells showed negative staining. The positivity of PCNA revealed no significant difference between non-recurrent (mean; 40.9%) and recurrent group (34.4%). The expression of c-fos oncogene was seen in 5 cases (100%) in recurrent group, and 8 cases (66.7%) in non-recurrent group, and no significant difference was seen. No significant difference of expression of TGF-alpha was seen in 5 cases (100%) in recurrent group and in 11 cases (91.7%) in non-recurrent group. The expression of TGF-beta in stromal cells was significantly higher in non-recurrent group (80%) compared to recurrent group (100%) (p<0.05). In DNA analysis out of 18 cases, 4 cases (22.2%) were aneuploidy and 14 cases (77.8%) were diploidy. Among 4 aneuploidy cases, 3 cases (75%) had no recurrence, and 1 case (25%) had metastasis to lung and expired. No significant difference of DNA ploidy pattern was seen between the recurrent and non-recurrent group.
CONCLUSION
Presence of more than 10 mitotic figures in 10 high power fields and less expression of TGF-beta are related to higher possibility of recurrence and it is suggested that the number of mitotic figure (more than 10/10HPF) and expression of TGF-beta could be helpful parameters in predicting recurrence of GCT.

Keyword

Bone; Giant cell tumor; PCNA; TGF-alpha & beta; Ploidy pattern

MeSH Terms

Aneuploidy
Carcinogenesis
Diploidy
DNA*
Giant Cell Tumor of Bone*
Giant Cell Tumors*
Giant Cells*
Hemorrhage
Lung
Necrosis
Negative Staining
Neoplasm Metastasis
Oncogenes
Ploidies
Prevalence
Prognosis
Proliferating Cell Nuclear Antigen*
Recurrence
Stromal Cells
Transforming Growth Factor alpha
Transforming Growth Factor beta
Transforming Growth Factors*
DNA
Proliferating Cell Nuclear Antigen
Transforming Growth Factor alpha
Transforming Growth Factor beta
Transforming Growth Factors
Full Text Links
  • JKCA
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr