Korean J Otolaryngol-Head Neck Surg.  1997 Jun;40(6):861-868.

Expression of TGF-alpha, p53 and PCNA in Laryngeal Dysplasia

• Choi JO
• Kim KH
• Chung K
• Choi K
• Yeom BW
• Min HK

Abstract

BACKGROUND: Transforming growth factor(TGF-alpha) is a polypeptide which is structurally related to epidermal growth factor(EGF) and binds to the epidermal growth factor receptor(EGFR). TGF-alpha utilizes EGFR to increase the activation of tyrosine kinase to involve in signal transduction of cellular growth. TGF-alpha synthesis occurs in a variety of neoplastic cells. p53 is a tumor-suppressor gene. There is a strong corrleation between immunohistochemical p53 positivity and p53 mutations in lung and laryngeal carcinoma. PCNA is expressed by cycling cells in late G1, S and G2 phase, and may be used to indicate the degree of cellular proliferation.
OBJECTIVES
To evaluate whether TGF-alpha, p53 and PCNA can be used as an indicator to malignant transformation of dysplasia in larynx or not.
MATERIALS AND METHODS
The authors investigated the TGF-alpha score, p53 immunoreactivity and PCNA proliferating index by immunohistochemical staining in 30 laryngeal dysplasia from 1992 to 1995.
RESULTS
Dysplasia with malignant transformation showed values of 5.46 for TGF-alpha, 29.2 for PCNA proliferating index and 37.5 for p53 immunoreactivity. Dysplasia without malignant transformation showed values of 1.88 for TGF-alpha, 8.6 for PCNA proliferating index and 4.5 for p53 immunoreactivity.
Conclusions
The results suggest that TGF-alpha, p53 and PCNA could be an useful indicator to predict the progression of laryngeal dysplasia.

Keyword

TGF-alpha; p53; Proliferating cell nuclear antigen; Laryngeal dysplasia

MeSH Terms

Cell Proliferation
Epidermal Growth Factor
G2 Phase
Larynx
Lung
Proliferating Cell Nuclear Antigen*
Protein-Tyrosine Kinases
Signal Transduction
Transforming Growth Factor alpha*
Epidermal Growth Factor
Proliferating Cell Nuclear Antigen
Protein-Tyrosine Kinases
Transforming Growth Factor alpha