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Human papillomavirus (HPV) DNA detection in uterine cervix cancer after radiation indicating recurrence: a systematic review and meta-analysis

Sabeena S, Kuriakose , Damodaran , Ravishankar N, Arunkumar

OBJECTIVE: The causal association of human papillomavirus (HPV) in uterine cervical cancer was well established and this oncogenic virus was reported to be a biomarker for overall recurrence and central...
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Epigenetic Changes in Asthma: Role of DNA CpG Methylation

Bae DJ, Jun JA, Chang HS, Park JS, Park CS

For the past three decades, more than a thousand of genetic studies have been performed to find out the genetic variants responsible for the risk of asthma. Until now, all...
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Effect of the Interaction Between Selenium and Zinc on DNA Repair in Association With Cancer Prevention

Yildiz A, Kaya Y, Tanriverdi O

Cancer is the most common cause of death worldwide. Annually, more than ten million new cancer cases are diagnosed, and more than six million deaths occur due to cancer. Nonetheless,...
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Role of DNA Methylation in the Development and Differentiation of Intestinal Epithelial Cells and Smooth Muscle Cells

Jorgensen , Ro S

  • KMID: 2454636
  • J Neurogastroenterol Motil.
  • 2019 Jul;25(3):377-386.
  • doi: 10.5056/jnm19077
The mammalian intestine contains many different cell types but is comprised of 2 main cell types: epithelial cells and smooth muscle cells. Recent in vivo and in vitro evidence has...
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Therapeutic Targeting of the DNA Damage Response Using an ATR Inhibitor in Biliary Tract Cancer

Nam AR, Jin MH, Park JE, Bang JH, Oh DY, Bang YJ

PURPOSE: The DNA damage response (DDR) is a multi-complex network of signaling pathways involved in DNA damage repair, cell cycle checkpoints, and apoptosis. In the case of biliary tract cancer...
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Does conventional freezing affect sperm DNA fragmentation?

Le MT, Nguyen TT, Nguyen TT, Nguyen TV, Nguyen TA, Nguyen QH, Cao TN

OBJECTIVE: Sperm cryopreservation has been widely used in assisted reproductive technology, as it offers great potential for the treatment of some types of male infertility. However, cryopreservation may result in...
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Clinical significance of cell-free DNA as a prognostic biomarker in patients with diffuse large B-cell lymphoma

Eskandari , Manoochehrabadi S, Pashaiefar H, Zaimy MA, Ahmadvand

BACKGROUND: Cell-free DNA (cfDNA) has the potential to serve as a non-invasive prognostic biomarker in some types of neoplasia. The investigation of plasma concentration of cfDNA may reveal its use...
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Respiratory Syncytial Virus Fusion Protein-encoding DNA Vaccine Is Less Effective in Conferring Protection against Inflammatory Disease than a Virus-like Particle Platform

Kwon YM, Hwang HS, Lee YT, Kim KH, Lee Y, Kim MC, Lee YN, Quan FS, Moore ML, Kang SM

Formalin-inactivated respiratory syncytial virus (RSV) vaccination causes vaccine-enhanced disease (VED) after RSV infection. It is considered that vaccine platforms enabling endogenous synthesis of RSV immunogens would induce favorable immune responses...
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Necessity of Guidelines for Research in Forensic Science: DNA Identification

Jung KW

Forensic science is an academic field that utilizes scientific knowledge and the conducting of research in legal procedures. Recently, legal science has been receiving more attention since the use of...
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The length of guide RNA and target DNA heteroduplex effects on CRISPR/Cas9 mediated genome editing efficiency in porcine cells

Lv J, Wu S, Wei R, Li Y, Jin J, Mu Y, Zhang Y, Kong Q, Weng X, Liu Z

The clustered regularly interspaced short palindrome repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a versatile genome editing tool with high efficiency. A guide sequence of 20 nucleotides (nt) is commonly...
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Cytochrome C Oxidase Subunit 1, Internal Transcribed Spacer 1, Nicotinamide Adenine Dinucleotide Hydrogen Dehydrogenase Subunits 2 and 5 of Clonorchis sinensis Ancient DNA Retrieved from Joseon Dynasty Mummy Specimens

Hong JH, Oh CS, Chai JY, Seo M, Shin DH

We analyzed Clonorchis sinensis ancient DNA (aDNA) acquired from the specimens of the Joseon mummies. The target regions were cytochrome C oxidase subunit 1 (CO1), internal transcribed spacer 1 (ITS1),...
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Effect of Sperm DNA Fragmentation on Embryo Quality in Normal Responder Women in In Vitro Fertilization and Intracytoplasmic Sperm Injection

Kim SM, Kim SK, Jee BC, Kim SH

PURPOSE: To investigate the associations between sperm DNA fragmentation (SDF) and embryo formation rate in normal responder women to in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI). MATERIALS AND METHODS: Fifty-three consecutive, fresh...
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Prospective study of the efficacy and utility of TP53 mutations in circulating tumor DNA as a non-invasive biomarker of treatment response monitoring in patients with high-grade serous ovarian carcinoma

Kim YM, Lee SW, Lee YJ, Lee HY, Lee JE, Choi EK

OBJECTIVE: Somatic TP53 mutation (TP53mut ) is a characteristic finding in high-grade serous ovarian cancer (HGSOC). The aim of this study was to assess the clinical efficacy and utility of TP53mut circulating...
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A Phase II Trial of Osimertinib in the Second-Line Treatment of Non-small Cell Lung Cancer with the EGFR T790M Mutation, Detected from Circulating Tumor DNA: LiquidLung-O-Cohort 2

Park CK, Cho HJ, Choi YD, Oh IJ, Kim YC

PURPOSE: Administering the best treatment after failure of epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI) therapy requires knowledge of resistance status. In this trial, treatment efficacy of osimertinib...
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Identification of Serial DNA Methylation Changes in the Blood Samples of Patients with Lung Cancer

Moon DH, Kwon SO, Kim WJ, Hong Y

BACKGROUND: The development of lung cancer results from the interaction between genetic mutations and dynamic epigenetic alterations, although the exact mechanisms are not completely understood. Changes in DNA methylation may...
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Effects of Pre-analytical Variables on Cell-free DNA Extraction for Liquid Biopsy

Jeong TD, Kim MH, Park S, Chung HS, Lee JW, Chang JH, Huh J

BACKGROUND: Extraction of cell-free DNA (cfDNA) is a key step for determining the quality of cfDNA-related molecular diagnostics. We evaluated the effect of sample containers and sample storage conditions on...
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Evaluation of sperm DNA fragmentation using multiple methods: a comparison of their predictive power for male infertility

Javed A, Talkad MS, Ramaiah MK

OBJECTIVE: The usual seminal profile has been customarily used for diagnosing male infertility based on an examination of semen samples. However, sperm DNA fragmentation has also been causally linked to...
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Evaluation of normal morphology, DNA fragmentation, and hyaluronic acid binding ability of human spermatozoa after using four different commercial media for density gradient centrifugation

Lee D, Jee BC

OBJECTIVE: Density gradient centrifugation (DGC) is frequently used to isolate high-motility fractions of spermatozoa. We compared the efficacy of four DGC media in terms of the percentage of morphologically normal...
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Advantages of Array Comparative Genomic Hybridization Using Buccal Swab DNA for Detecting Pallister-Killian Syndrome

Bertini V, Gana S, Orsini A, Bonuccelli A, Peroni D, Angelo V

No abstract available.
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Quantification of Cell-Free DNA: A Comparative Study of Three Different Methods

Jeon K, Lee J, Lee JS, Kim M, Kim HS, Kang HJ, Lee YK

BACKGROUND: Cell-free DNA (cfDNA) provides unique potential as a biomarker for cancer patients or in the field of prenatal care. Following extraction from plasma, cfDNA should be quantified before downstream...
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