J Gynecol Oncol.  2010 Dec;21(4):255-261. 10.3802/jgo.2010.21.4.255.

Apoptosis-related mRNA expression profiles of ovarian cancer cell lines following cisplatin treatment

Affiliations
  • 1Department of Obstetrics and Gynecology, St. Vincent's Hospital, The Catholic University of Korea, Suwon, Korea.
  • 2School of Inter-Disciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology (POSTECH), Pohang, Korea. kchoi@postech.ac.kr
  • 3Division of Molecular and Life Sciences, Department of Life Science, Pohang University of Science and Technology (POSTECH), Pohang, Korea.

Abstract


OBJECTIVE
The aim of this study was to identify apoptosis-related genes of ovarian cancer cell lines following cisplatin treatment.
METHODS
We used IC50 values and fluorescence-activated cell sorting analysis to compare cell death in 2 ovarian cancer cell lines, namely, SKOV-3 and OVCAR-3, upon treatment with cisplatin. Moreover, the change in transcriptional levels of apoptosis-associated genes was measured with a dendron-modified DNA microarray.
RESULTS
The protein levels for the up-regulated genes in each cell line were validated to identify the molecules that may determine the cellular behavior of cisplatin resistance. Eight genes were over-expressed in the 2 cell lines. The cisplatin-induced up-regulation of DAD1 in transcriptional and protein levels contributed to the cisplatin resistance of OVCAR-3, and the up-regulation of FASTK and TNFRSF11A in SKOV-3 resulted in its higher sensitivity to cisplatin than that of OVCAR-3.
CONCLUSION
In the present study, we have identified a set of genes responsible for apoptosis following cisplatin treatment in ovarian cancer cell lines. These genes may give information about the understanding of cisplatin-induced apoptosis in ovarian cancer.

Keyword

Ovarian cancer cell lines; Cisplatin; Apoptosis; Dendron-modified DNA microarray

MeSH Terms

Apoptosis
Cell Death
Cell Line
Cisplatin
DNA
Flow Cytometry
Inhibitory Concentration 50
Ovarian Neoplasms
RNA, Messenger
Up-Regulation
Cisplatin
DNA
RNA, Messenger
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