Exp Neurobiol.  2010 Jun;19(1):39-48. 10.5607/en.2010.19.1.39.

Normal Adult Hippocampal Neurogenesis in SRG3-overexpressing Transgenic Mice

Affiliations
  • 1Department of Biochemistry and Molecular Biology, Hanyang University College of Medicine, Seoul 133-791, Korea. hyeonson@hanyang.ac.kr
  • 2Department of Pathology, Hanyang University College of Medicine, Seoul 133-791, Korea.
  • 3Department of Biological Sciences, Institute of Molecular Biology and Genetics and Research Center for Functional Cellulomics, Seoul National University, Seoul 151-742, Korea.

Abstract

SRG3 (SWI3-related gene) is a core subunit of mouse SWI/SNF complex and is known to play a critical role in stabilizing the SWI/SNF complex by attenuating its proteasomal degradation. SWI/SNF chromatin remodeling complex is reported to act as an important endogenous regulator in the proliferation and differentiation of mammalian neural stem cells. Because limited expression of SRG3 occurs in the brain and thymus during mouse embryogenesis, it was hypothesized that the altered SRG3 expression level might affect the process of adult hippocampal neurogenesis. Due to the embryonic lethality of homozygous knockout mice, this study focuses on dissecting the effect of overexpressed SRG3 on adult hippocampal neurogenesis. The BrdU incorporation assay, immunostaing with neuronal markers for each differentiation stage, and imunoblotting analysis with intracellular molecules involved in survival in adult hippocampal neurogenesis found no alteration, suggesting that the overexpression of SRG3 protein in mature neurons had no effect on the entire process of adult hippocampal neurogenesis including proliferation, differentiation, and survival.

Keyword

SRG3 (SWI3-related gene); SWI/SNF complx; adult hippocampal neurogenesis; proliferation; differentiation; survival
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