Korean J Anat.  1999 Dec;32(6):831-836.

Human genomic DNA isolation and chromosomal localization of fetal brain cDNA (FB174)

• Park SY
• Kang YH
• Lee HL
• Kim H
• Park SH

Abstract

Eventhough surmountable amounts of genes are being cloned and a number of methods are being developed by human genome project, it's not easy to predict possible functions of genes and determine the chromosomal locations of genes. In this experiment, cDNA pool was made from 18 weeks old human fetal brain and analyzed the sequences. FB174 clone was chosen, in situ hybridization histochemistry was performed on developing and adult rat tissue section to observe the tissue specificity and developmental expression of this gene. To observe the chromosomal location of FB174 clone, the genomic DNA from human genomic library was isolated and fluorescence in situ hybridization was carried out. By sequencing and sequence search with GenBank data it was revealed that cloned FB174 cDNA was quite similar to translationally controlled tumor protein which is known to locate to human chromosome 13q14. The expression of FB174 mRNA was not detected in rat tissue sections by in situ hybridization histochemistry. Fluorescence in situ hybridization using biotin labeled FB174 probe resulted in specific labeling of human chromosome 7q22. These results and high sequence homology of FB174 to known translationally controlled tumor protein suggest that FB174 clone may be a new translationally controlled tumor protein-related gene.

Keyword

Human fetal brain; Genomic DNA; Sequencing; In situ hybridization; Fluorescence in situ hybridization

MeSH Terms

Adult
Animals
Biotin
Brain*
Chromosomes, Human
Clone Cells
Databases, Nucleic Acid
DNA*
DNA, Complementary*
Fluorescence
Genomic Library
Human Genome Project
Humans*
In Situ Hybridization
Organ Specificity
Rats
RNA, Messenger
Sequence Homology
Biotin
DNA
DNA, Complementary
RNA, Messenger