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Cloning and sequencing of p33 in a Korean isolate of Theileria sergenti

Kang SW, Choi EJ, Kweon CH

The gene encoding the 33 kDa piroplasm surface protein of Theileria sergenti isolated in Korea was cloned and the nucleotide sequence was determined by dideoxy chain termination method. The cloned...
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Sequence Analysis and Expression of the VP7 Gene of G1 Rotavirus Isolated from an Infant in Korean

Song MO, Park CM, In SJ, Kim KJ, Chung SI, Choi CS, Kim WY, Lim IS

  • KMID: 1551993
  • J Korean Soc Virol.
  • 1998 Sep;28(3):247-265.
To determine the sequence and expression of the VP7 gene of Korean isolates (CAU-9), viral RNA was purified and used for cDNA amplification by RT-PCR. The VP7 cDNA was cloned,...
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Differential diagnosis of Salmonella gallinarum and S. pullorum using PCR-RELP

Park MK, Choi KS, Kim MC, Chae JS

  • KMID: 1119218
  • J Vet Sci.
  • 2001 Dec;2(3):213-219.
Salmonellosis in poultry of Korea is a significant health problem, which causes substantial economic losses. The most common causative agents of chicken salmonellosis ar S. gallinarum and S. pullorum. Traditional...
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Studies of cocktail therapy with multiple cytokines for neoplasia or infectious disease of the dog I. cDNA cloning of canine IL-3 and IL-6

Shin IS, Kim HR, Nam MJ, Youn HY

  • KMID: 1103518
  • J Vet Sci.
  • 2001 Aug;2(2):115-120.
This paper describes the cloning and sequence analysis of the cDNAs encoding the canine homologues of interleukin-3 (IL-3) and interleukin-6 (IL-6). The coding sequences for canine IL-3 and IL-6 were...
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A New Approach to Find Orthologous Proteins Using Sequence and Protein-Protein Interaction Similarity

Kim MK, Seol YJ, Park HS, Jang SH, Shin HC, Cho KH

  • KMID: 1469572
  • Genomics Inform.
  • 2009 Sep;7(3):141-147.
Developed proteome-scale ortholog and paralog prediction methods are mainly based on sequence similarity. However, it is known that even the closest BLAST hit often does not mean the closest neighbor....
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Genetic characterization of porcine circovirus-2 field isolates from PMWS pigs

Kim JH, Lyoo YS

  • KMID: 1101572
  • J Vet Sci.
  • 2002 Mar;3(1):31-39.
PMWS is a new emerging disease in swine herds worldwide. Field isolates of PCV-2, a putative major causative agent of PMWS, were isolated and genetically characterized. Viral genome of two...
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Allergenicity of Genetically Modified Crops

Kim HY, Kim JH

  • KMID: 2314506
  • Pediatr Allergy Respir Dis.
  • 2007 Sep;17(3):166-172.
During the period 1996 to 2006, 51 countries have granted regulatory approvals for genetically modified (GM) crops for import for food and feed use and for release into the environment....
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Genomic Organization of ancop Gene for alpha-COP Homolog from Aspergillus nidulans

Lee HH, Chae SK, Kim JY, Maeng PJ, Park HM

  • KMID: 2312327
  • Mycobiology.
  • 2000 Dec;28(4):171-176.
We have cloned a alpha-COP homolog, ancop, from Aspergillus nidulans by colony hybridization of chromosome specific library using alpha-COP homologous fragment as a probe. The probe DNA was amplified with...
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Purification and cloning of glyoxalase II from rat liver

Cho MY, Bae CD, Park JB, Lee TH

  • KMID: 754988
  • Exp Mol Med.
  • 1998 Mar;30(1):53-57.
Glyoxalase (GLO) II, which is a component of GLO system and catalyze the conversion of S-lactoyl-glutathione to D-lactate, was purified 1488 fold from rat liver by two steps of Affigel blue and...
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Correlation of Virologic Property and Phylogenetic Analysis of Hantaan Viruses Isolated from Patients and Reservoirs in Korea

Jung YT, Lee SR, Paik SY, Kim GR

  • KMID: 1940941
  • J Korean Soc Virol.
  • 1998 Mar;28(1):11-20.
Twenty isolates of Hantavirus were isolated from patients and reserovirs from 1988 to 1994 in Korea. Isolation rate was 1.9% (10/538) in patients, 6.2% (5/81) in Apodemus sp., 2.6% (1/38)...
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Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand

Pohuang T, Chansiripornchai N, Tawatsin A, Sasipreeyajan J

Thirteen field isolates of infectious bronchitis virus (IBV) were isolated from broiler flocks in Thailand between January and June 2008. The 878-bp of the S1 gene covering a hypervariable region...
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Whole Mitochondrial Genome Sequence of an Indian Plasmodium falciparum Field Isolate

Tyagi S, Pande V, Das A

Mitochondrial genome sequence of malaria parasites has served as a potential marker for inferring evolutionary history of the Plasmodium genus. In Plasmodium falciparum, the mitochondrial genome sequences from around the...
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Isolation and Characterization of Avian Metapneumovirus from Broiler Breeder Chickens in Korea

Choi KS, Jeon WJ, Park MJ, Lee EK, Kwon JH

Avian metapneumovirus (AMPV) is an emerging pathogen causing respiratory and reproductive illness in poultry worldwide. To demonstrate the presence of AMPV in domestic chickens in Korea, we attempted to isolate...
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Genetic Variation in the NSP4 Gene of Human Rotavirus Isolated in Seoul

Cho SL, Ahn JH, Kim K, Chung SI, Lim I, Kim W

The nonstructural protein 4 (NSP4) of rotavirus encoded by gene 10, plays an important role in rotavirus pathogenicity. In this study, NSP4 gene sequences of human rotaviruses circulating in Seoul,...
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A case study of apple seed and grape allergy with sensitisation to nonspecific lipid transfer protein

Murad A, Katelaris , Baumgart K

Lipid transfer proteins can be an important cause of allergy given their stability and high degree of protein sequence homology. We describe the case of a child who developed two...
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Differentially expressed genes of Acanthamoeba castellanii during encystation

Moon EK, Chung DI, Hong YC, Kong HH

To examine the expressed gene profile during encystation of Acanthamoeba castellanii Castellani, we used differentially expressed gene (DGE) screening by RT-PCR with 20 sets of random primers. From this analysis,...
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Cloning and expression of trypsin-encoding cDNA from Blattella germanica and its possibility as an allergen

Ock MS, Kim BJ, Kim SM, Byun KH

In this study, the trypsin gene (bgtryp-1) from the German cockroach, Blattella germanica, was cloned via the immunoscreening of patients with allergies to cockroaches. Nucleotide sequence analysis predicted an 863...
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Sequence analysis of the S1 glycoprotein gene of infectious bronchitis viruses: identification of a novel phylogenetic group in Korea

Jang JH, Sung HW, Song CS, Kwon HM

Twelve Korean infectious bronchitis viruses (IBVs) were isolated in the field from chickens suspected of being carriers of infectious bronchitis between 2001 and 2003. The S1 glycoprotein genes of these...
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Molecular cloning of clathrin assembly protein gene (rCALM) and its differential expression to AP180 in rat brain

Kim HL, Lee SC

  • KMID: 1029926
  • Exp Mol Med.
  • 1999 Dec;31(4):191-196.
Binding of clathrin assembly protein to clathrin triskelia induces their assembly into clathrin-coated vesicle (CCV) in neurons. The clathrin assembly protein gene (rCALM) was cloned from rat brain...
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Characterization of Bacterial Community Contaminating Floor of A Hot and Dry Sauna

Lee JY, Park DH

The purpose of this study was to examine profile of bacteria contaminated in a dry sauna. Bacteria sampled from the floor of the dry sauna (75~80degrees C) were separated and...
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