Korean J Obstet Gynecol.  2003 Jan;46(1):38-43.

Chromosomal gains and losses in primary ovarian carcinomas by comparative genomic hybridization

Affiliations
  • 1Department of Obstetrics and Gynecology, College of Medicine, Korea University, Korea.
  • 2Sewha Pediatric Clinic, Seoul, Korea.

Abstract


OBJECTIVE
Comparative genomic hybridization was performed to evaluate DNA sequence copy number changes in human ovarian carcinomas from paraffin-embedded tissue blocks.
PATIENTS AND METHODS
DNA from 20 cases of primary ovarian carcinomas underwent comparative genomic hybridization to evaluate the extent of genetic gains or losses in a test sample.
RESULTS
In thirteen cases of 20 samples, varying degree of genetic imbalances was observed. Of the remaining 7 cases, two revealed normal, five failed to yield a result. Most common genetic imbalances are 8q22.2-q24 site amplification and 12p site amplification, where c-myc gene and k-ras gene respectively are included. Second most common site of genetic imbalance is 7p21-pter site deletion.
CONCLUSION
Our results have shown many chromosomal alterations in human ovarian carcinomas, and these sites are known previously as oncogene or tumor-suppression gene, and some sites are not known specific cancer associated sites. Our data can be useful for screening chromosomal changes and molecular mechanism of human ovarian carcinogenesis.

Keyword

ovarian cancer; comparative genomic hybridization; FISH; chromosomal change

MeSH Terms

Base Sequence
Carcinogenesis
Comparative Genomic Hybridization*
DNA
Genes, myc
Genes, ras
Humans
Mass Screening
Oncogenes
Ovarian Neoplasms
DNA
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