Exp Mol Med.  2000 Dec;32(4):187-192.

Characterization of an unusual variant mRNA of human lysosomal alpha-mannosidase

Affiliations
  • 1Laboratory of Pathology and Center for Comparative Medical Genetics, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104, USA.

Abstract

Lysosomal alpha-mannosidase (EC 3.2.1.24) is an exoglycosidase in the glycoprotein degradation pathway and is encoded by a 3.0 kb cDNA. A 2.3 kb cDNA from a minor species of HeLa cell mRNA was discovered by RT-PCR cloning. Southern blotting and PCR analysis of the HeLa cell genomic DNA showed that the 2.3 kb message was encoded by the lysosomal alpha-mannosidase gene. Sequence comparison of the cDNA with the corresponding genomic DNA indicated that the 2.3 kb message was generated by an unusual intra-exonic joining event.

Keyword

lysosomal a-mannosidase; intron sequences; mRNA; splice signal sequence

MeSH Terms

*Alternative Splicing
Base Sequence
DNA, Complementary/genetics
Exons
Hela Cells
Human
Lysosomes/*enzymology
Mannosidases/*genetics
Molecular Sequence Data
RNA, Messenger/*genetics
Reverse Transcriptase Polymerase Chain Reaction
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