Korean J Physiol Pharmacol.  2007 Aug;11(4):149-154.

Identification of Differentially Expressed Genes in Murine Hippocampus by Modulation of Nitric Oxide in Kainic Acid-induced Neurotoxic Animal Model

Affiliations
  • 1Department of Pharmacology, College of Medicine, Kangwon National University, Chuncheon 200-701, Korea. ksslsy@kangwon.ac.kr
  • 2Department of Anesthesiology and Pain Medicine, College of Medicine, Hallym University, Chuncheon 200-704, Korea.

Abstract

Kainic acid (KA) causes neurodegeneration, but no consensus has been reached concerning its mechanism. Nitric oxide may be a regulator of the mechanism. We identified differentially expressed genes in the hippocampus of mice treated with kainic acid, together with or without L-NAME, a nonselective nitric oxide synthase inhibitor, using a new differential display PCR method based on annealing control primers. Eight genes were identified, including clathrin light polypeptide, TATA element modulatory factor 1, neurexin III, ND4, ATPase, H+ transporting, V1 subunit E isoform 1, and N-myc downstream regulated gene 2. Although the functions of these genes and their products remain to be determined, their identification provides insight into the molecular mechanism(s) involved in KA-induced neuronal cell death in the hippocampal CA3 area.

Keyword

Excitotoxicity; Kainic acid; L-NAME; Differential display PCR; Hippocampus

MeSH Terms

Animals*
Cell Death
Clathrin
Consensus
Hippocampus*
Kainic Acid
Mice
Models, Animal*
Neurons
NG-Nitroarginine Methyl Ester
Nitric Oxide Synthase
Nitric Oxide*
Polymerase Chain Reaction
Proton-Translocating ATPases
Clathrin
Kainic Acid
NG-Nitroarginine Methyl Ester
Nitric Oxide
Nitric Oxide Synthase
Proton-Translocating ATPases
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