Exp Mol Med.  1998 Dec;30(4):177-185.

Isolation and characterization of brain-specific transglutaminases from rat

• Kwak SJ
• Kim SY
• Kim YS
• Song KY
• Kim IG
• Park SC

Abstract

The relevance of transglutaminases with neural function and several disorders has been emphasized recently. Especially, many polypeptides associated with neurodegenerative diseases are suggested to be putative transglutaminase substrates such as beta amyloid protein of Alzheimer's disease, microtubule-associated proteins and neurofilaments, etc. In addition, the CAG repeated gene products with probable polyglutamine tract, putative transglutaminase substrates, were identified in several neurodegenerative disorders. However, the identity of the brain transglutaminase has not been confirmed, because of enzymic stability and low activity. In the present experiment, we have isolated brain-specific transglutaminases, designated as TGase NI and TGase NII, which are different from other types of transglutaminases in respects of molecular weights (mw. 45 kDa, 29 kDa respectively), substrate affinity, elution profile on ion-exchange chromatography, sensitivity to proteases and ethanol, and immunological properties. The enzymes were localized specifically in the brain tissues but not in the liver tissue. And neural cells such as pheochromocytoma cell, glioma cell, primary neuronal and glial cells were shown to be enriched with TGase NI and TGase NII. The possible biological roles of the enzymes were discussed not only on the aspect of crosslinking activity but also of signal transducing capacity of the enzyme in the brain.

Keyword

TGase NI; TGaase NII; brain; rat; localization

MeSH Terms

Animal
Astrocytes/enzymology
Blotting, Western
Brain/enzymology*
Calcium/metabolism
Chromatography, Ion Exchange
Endopeptidases/pharmacology
Enzyme Stability
Ethanol/pharmacology
Glioma
Immunoblotting
Immunohistochemistry
Male
Molecular Weight
Neurons/enzymology
PC12 Cells
Protein-Glutamine gamma-Glutamyltransferase/isolation & purification*
Protein-Glutamine gamma-Glutamyltransferase/immunology
Protein-Glutamine gamma-Glutamyltransferase/chemistry*
Rats
Rats, Sprague-Dawley
Trypsin/pharmacology
Tumor Cells, Cultured