J Korean Acad Oral Health.  2020 Mar;44(1):13-19. 10.11149/jkaoh.2020.44.1.13.

Antibacterial effect of different concentrations of Galla Chinensis extract on cariogenic bacteria in a biofilm model

Affiliations
  • 1Dental Research Institution, School of Dentistry, Seoul National University, Seoul, Korea.
  • 2Department of Preventive & Social Dentistry, School of Dentistry, Seoul National University, Seoul, Korea. jjbh@snu.ac.kr

Abstract

OBJECTIVES: Galla chinensis inhibited the adherence of planktonic oral bacteria and acid production by cariogenic bacteria. However, little is known about the relevant conditions of Galla Chinensis extract (GCE) exposure time and concentration and the effect of GCE on the structural and functional activity of cariogenic bacteria. The antibacterial effects of natural G. Chinensis extract on S. mutans , S. sanguinis, and S. oralis biofilms were evaluated in vitro.
METHODS
Biofilms formed on glass surfaces were treated with different concentrations of GCE at different exposure times. The effects were assessed by examining the bactericidal activity, acidogenesis, minimum inhibitory concentration, and morphology.
RESULTS
There was a statistically significant difference in the bacterial growth inhibition depending on the concentration of the GCE, with bacterial growth being inhibited as the concentration of GCE increased. A concentration of 1.0 mg/ml GCE had similar bactericidal effects against S. mutans and S. oralis biofilms to those produced by 2.0 mg/ml CHX. In the 1.0 mg/ml GCE group, incomplete septa were also observed in the outline of the cell wall, together with disruption of the cell membrane. In addition, there was also a slight exudation of the intracellular content from the bacteria in the 1.0 mg/ml GCE and 2 mg/ml CHX groups.
CONCLUSIONS
These results indicate that GCE inhibits the growth of S. mutans, S. sanguinis, and S. oralis with increasing concentrations. It alters the microstructure of S. mutans biofilms. These results suggest that GCE might be a useful anti-bacterial agent for preventing dental caries.

Keyword

Streptococcus mutans; Galla Chinensis; Biofilms; Antibacterial effects
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