J Korean Acad Oral Health.  2016 Mar;40(1):3-8. 10.11149/jkaoh.2016.40.1.3.

Identification and partial purification of antibacterial compounds against Streptococcus mutans from Galla Rhois

Affiliations
  • 1Department of Preventive & Public Health Dentistry, Chonnam National University School of Dentistry, Gwangju, Korea. sjhong@chonnam.ac.kr
  • 2Department of Oral Microbiology, Chonnam National University School of Dentistry, Gwangju, Korea.
  • 3Dental Science Research Institute, Chonnam National University School of Dentistry, Gwangju, Korea.

Abstract

OBJECTIVES: This study aims to identify and partially purify antibacterial compounds against Streptococcus mutans from Galla Rhois extract.
METHODS
Galla Rhois was extracted with n-hexane or ethanol and concentrated in a rotary evaporator. The antibacterial effect of the Galla Rhois extract against S. mutans was determined by the paper discdiffusion method with n-hexane, ethanol, methanol, ethyl acetate, dimethyl sulfoxide (DMSO), acetone, and distilled water as the solvents. The active compounds were purified by partition chromatography, thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC).
RESULTS
The antibacterial effect of the n-hexane extract was more effective against S. mutans than the ethanol extract (P<0.05). The antibacterial component of Galla Rhois was partially purified using partition chromatography and HPLC, and the antibacterial activity was confirmed.
CONCLUSIONS
The partially purified component of Galla Rhois showed strong antibacterial effect against S. mutans. These results confirm that the antibacterial compounds of Galla Rhois can be used for the prevention of dental caries.

Keyword

Antibacterial compounds; Column chromatography; Galla Rhois; Partition chromatography; Streptococcus mutans; Thin-layer chromatography
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