J Korean Acad Oral Health.  2016 Mar;40(1):3-8. 10.11149/jkaoh.2016.40.1.3.

Identification and partial purification of antibacterial compounds against Streptococcus mutans from Galla Rhois

  • 1Department of Preventive & Public Health Dentistry, Chonnam National University School of Dentistry, Gwangju, Korea. sjhong@chonnam.ac.kr
  • 2Department of Oral Microbiology, Chonnam National University School of Dentistry, Gwangju, Korea.
  • 3Dental Science Research Institute, Chonnam National University School of Dentistry, Gwangju, Korea.


This study aims to identify and partially purify antibacterial compounds against Streptococcus mutans from Galla Rhois extract.
Galla Rhois was extracted with n-hexane or ethanol and concentrated in a rotary evaporator. The antibacterial effect of the Galla Rhois extract against S. mutans was determined by the paper discdiffusion method with n-hexane, ethanol, methanol, ethyl acetate, dimethyl sulfoxide (DMSO), acetone, and distilled water as the solvents. The active compounds were purified by partition chromatography, thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC).
The antibacterial effect of the n-hexane extract was more effective against S. mutans than the ethanol extract (P<0.05). The antibacterial component of Galla Rhois was partially purified using partition chromatography and HPLC, and the antibacterial activity was confirmed.
The partially purified component of Galla Rhois showed strong antibacterial effect against S. mutans. These results confirm that the antibacterial compounds of Galla Rhois can be used for the prevention of dental caries.


Antibacterial compounds; Column chromatography; Galla Rhois; Partition chromatography; Streptococcus mutans; Thin-layer chromatography

MeSH Terms

Chromatography, High Pressure Liquid
Chromatography, Liquid
Chromatography, Thin Layer
Dental Caries
Dimethyl Sulfoxide
Streptococcus mutans*
Dimethyl Sulfoxide


  • Fig. 1. Clear zone diameter of S. mutans in extractant solution of Galla Rhois extract.

  • Fig. 2. Growth inhibitory activity of n-hexan extract from Galla Rhois aginst S. mutans by paper disc diffusion method after partition chromatography (a: n-hexan, b: Galla Rhois n-hexan extract, c: n-hexan fraction of Galla Rhois n-hexan extract, d: ethanol fraction of Galla Rhois n-hexan extract).

  • Fig. 3. TLC pattern of the n-hexan extract from Galla Rhois.

  • Fig. 4. Growth inhibitory activity of n-hexan extract from Galla Rhois aginst S. mutans by paper disc diffusion method after TLC (a: fraction S1, b: fraction S2, c: n-hexan, d: fraction S3, e: fraction S4, f: n-hexan).

  • Fig. 5. Chromatogram of n-hexan extract of Galla Rhois by Column chromatography.

  • Fig. 6. Growth inhibitory activity of n-hexan extract from Galla Rhois aginst S. mutans by paper disc diffusion method after Column chromatography (a: n-hexan, b: fr. 22, c: n-hexan, d: fr. 23, e: n-hexan, f: fr. 24).



1. Marsh P, Martin M. Oral microbiology. 4th ed.Oxford: Wright;1999. p. 82–103.
2. Bakri IM, Douglas CW. Inhibitory effect of garlic extract on oral bacteria. Arch Oral Biol. 2005; 50:645–651.
3. Islam B, Khan SN, Haque I, Alam M, Mushfiq M, Khan AU. Novel anti-adherence activity of mulberry leaves: inhibition of Streptococcus mutans biofilm by 1-deoxynojirimycin isolated from Morus alba. J Antimicrob Chemother. 2008; 62:751–757.
4. Naderi NJ, Niakan M, Kharazi Fard MJ, Zardi S. Antibacterial activity of iranian green and black tea on Streptococcus mutans: An in vitro study. J Dent. 2011; 8(2):55–59.
5. Xu X, Zhou XD, Wu CD. The tea catechin epigallocatechin gallate suppresses cariogenic virulence factors of Streptococcus mutans. Antimicrob Agents Chemother. 2011; 55(3):1229–1236.
6. An BJ. Effect of inhibition on glucosyltransferase and antimicrobial activity of polyphenol fraction of gallnut and red grape husk. Kor J Postharvest Sci Technol. 2001; 8:217–223.
7. Cha BC, Lee SB. Antioxidative and free radical scavenging effects of Rhus javanica Linne. Kor J Medicinal Crop Sci. 1998; 6(3):181–187.
8. Ahn YJ, Lee CO, Kweon JH, Ahn JW, Park JH. Growth-inhibitory effects of Galla Rhois-derived tannins on intestinal bacteria. J Appl Microbiol. 1998; 84:439–443.
9. Xie Q, Li JY, Zuo YL, Zhou XD. The effect of galla chinensis on the growth of cariogenic bacteria in vitro. Hua Xi Kou Qiang Yi Xue Za Zhi. 2005; 23:82–84.
10. Cho MJ, Hong SJ, Choi CH, Jeong SS. Effects of dentifrice containing extract of Galla Rhois or Psoralea corylifolia on inhibition of plaque formation. J Korean Acad Oral Health. 2005; 29(2):141–152.
11. Lee YS, Han OK, Bae MJ, Kim KJ, Shin SW, Lee SK, et al. Antimicrobial and anticancer effects of Galla Rhois on pathogens isolated from oral and KB human oral epidermoid carcinoma cells. Korean J Oriental Physiology & Pathology. 2003; 17(6):1427–1432.
12. Tian F, Li B, Ji B, Yang J, Zhang G, Chen Y, et al. Antioxidant and antimicrobial activities of consecutive extracts from Galla chinensis: The polarity affects the bioactivities. Food Chem. 2009; 113:173–179.
13. Choi JG, Kang OH, Lee YS, Oh YC, Chae HS, Jang HJ, et al. In vitro activity of methyl gallate isolated from galla rhois alone and in combination with ciprofloxacin against clinical isolates of salmonella. J Microbiol Biotechnol. 2008; 18(11):1848–1852.
14. Lee JJ, Bae JH, Kim DH, Lim JJ, Kim DG, Lee HJ, et al. Intracellular replication inhibitory effects of Galla Rhois ethanol extract for Brucella abortus infection. J Ethnopharmacol. 2011; 18. 138(2):602–609.
15. Park U, Chang DS, Cho HR. Screening of Antimicrobial Activity for Medicinal Herb Extracts. J Korean Soc Food Nutr. 1992; 21:91–96.
16. Yu YE, Park EY, Jung DH, Byun SH, Kim SC, Park SM. Antibacterial activity of oriental medicinal herb extracts against skin pathogens. J Life Sci. 2010; 20(7):1143–1150.
17. Elizabeth Peninisi, A mouthful of microbes. Science. 2005; 307:1899–1901.
18. Cem A. Gürgan, Eylem Zaim, Işıl Bakirsoy, Emel Soykan. Short-term side effects of 0.2% alcohol-free chlorhexidine mouthrinse used as an adjunct to non-surgical periodontal treatment: A double-blind clinical study. J Periodontol. 2006; 77(3):370–384.
19. Flötra L, Gjermo PER, Rölla G, Waerhaug J. Side effects of chlorhexidine mouth washes. Eur J Oral Sci. 1971; 79:119–125.
20. Flötra L. Different modes of chlorhexidine application and related local side effects. J Periodontal Res. 1973; 8:41–44.
21. Kang MS, Oh JS, Kang IC, Hong SJ, Choi CH. Inhibitory effect of methyl gallate and gallic acid on oral bacteria. J Microbiol. 2008; 46(6):744–750.
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