Korean J Physiol Pharmacol.  2020 Mar;24(2):149-156. 10.4196/kjpp.2020.24.2.149.

Inhibition of melanogenesis by sodium 2-mercaptoethanesulfonate

Affiliations
  • 1R&D Unit, AmorePacific Corporation, Yongin 17074, Korea.
  • 2Department of Dermatology, Chung-Ang University College of Medicine, Seoul 06974, Korea. beomjoon@cau.ac.kr
  • 3Department of Medicine, Graduate School, Chung-Ang University, Seoul 06974, Korea.

Abstract

Sodium 2-mercaptoethanesulfonate (mesna) is a protective agent that is widely used in medicine because of its antioxidant effects. Recently, reactive oxygen species (ROS) were shown to increase pigmentation. Thus, ROS scavengers and inhibitors of ROS production may suppress melanogenesis. Forkhead box-O3a (FoxO3a) is an antimelanogenic factor that mediates ROS-induced skin pigmentation. In this study, we aimed to investigate the whitening effect of mesna and the signaling mechanism mediating this effect. Human melanoma (MNT-1) cells were used in this study. mRNA and protein expression were measured by real-time quantitative PCR and Western blotting analysis to track changes in FoxO3a-related signals induced by mesna. An immunofluorescence assay was performed to determine the nuclear translocation of FoxO3a. When MNT-1 melanoma cells were treated with mesna, melanin production and secretion decreased. These effects were accompanied by increases in FoxO3a activation and nuclear translocation, resulting in downregulation of four master genes of melanogenesis: MITF, TYR, TRP1, and TRP2. We found that mesna, an antioxidant and radical scavenger, suppresses melanin production and may therefore be a useful agent for the clinical treatment of hyperpigmentation disorders.

Keyword

Antioxidants; Hyperpigmentation; Mesna; Skin lightening preparations

MeSH Terms

Antioxidants
Blotting, Western
Down-Regulation
Fluorescent Antibody Technique
Humans
Hyperpigmentation
Melanins
Melanoma
Mesna*
Negotiating
Pigmentation
Polymerase Chain Reaction
Reactive Oxygen Species
RNA, Messenger
Skin Lightening Preparations
Skin Pigmentation
Sodium*
Antioxidants
Melanins
Mesna
RNA, Messenger
Reactive Oxygen Species
Skin Lightening Preparations
Sodium
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