Immune Netw.  2019 Aug;19(4):e29. 10.4110/in.2019.19.e29.

Evaluation of Protective Immunity of Peptide Vaccines Composed of a 15-mer N-terminal Matrix Protein 2 and a Helper T-Cell Epitope Derived from Influenza A Virus

Affiliations
  • 1Department of Pharmacy, Korea University College of Pharmacy, Sejong 30019, Korea.
  • 2Bionano Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Korea. mklee@kribb.re.kr
  • 3Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, Korea University of Science and Technology (UST), Daejeon 34113, Korea.

Abstract

The matrix protein 2 of influenza A virus (IFAV) has a relatively conserved ectodomain (M2e) composed of 23 amino acids, and M2e-based vaccines have been suggested to induce broad protective immunity in mice. In this study, we investigated whether N-terminal sequence of M2e (nM2e)-based vaccines with more conserved nM2e could induce influenza viral neutralizing activity. We constructed linear peptide vaccines with an nM2e sequence for PR8 virus (nM2Pr) connected to a probable 17-mer IFAV-derived helper T-cell epitope (ThE: T1, T2, or T3) at its N- or C-terminus. The peptide vaccines induced significant production of nM2e Abs regardless of either type or location of the ThE-epitope in BALB/c mice, while only T3 was effective in C57BL/6 mice. The Abs against nM2Pr-T3 elicited broader binding affinities to the nM2e peptides derived from various IFAVs than those against T3-nM2Pr. In addition, the nM2e-based vaccines efficiently protected the immunized mice from the lethal challenge of PR8 virus. These results suggest that the more conserved nM2e without cysteine will be useful for development of universal peptide vaccines than M2e.

Keyword

Influenza Vaccines; M2 protein, Influenza A virus; Vaccines, Subunit; ELISA; Antibodies, Neutralizing

MeSH Terms

Amino Acids
Animals
Antibodies, Neutralizing
Cysteine
Enzyme-Linked Immunosorbent Assay
Influenza A virus*
Influenza Vaccines
Influenza, Human*
Mice
Peptides
T-Lymphocytes, Helper-Inducer*
Vaccines
Vaccines, Subunit*
Amino Acids
Antibodies, Neutralizing
Cysteine
Influenza Vaccines
Peptides
Vaccines
Vaccines, Subunit
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