J Bacteriol Virol.  2017 Jun;47(2):87-95. 10.4167/jbv.2017.47.2.87.

Replication of Recombinant Flock House Virus RNA Encapsidated by Turnip Yellow Mosaic Virus Coat Proteins in Nicotiana benthamiana

• Kim D
• Cho TJ

• KMID: 2384411
• DOI: http://doi.org/10.4167/jbv.2017.47.2.87

Abstract

It was previously observed that recombinant flock house virus (FHV) RNA1 was efficiently packaged into turnip yellow mosaic virus (TYMV), provided that the TYMV coat protein (CP) sequence was present at the 3"²-end. FHV RNA encapsidated by TYMV CPs also had a four-nucleotide extension at the 5"²-end. Since even a short extension at the 5"²- and 3"²-ends of FHV RNA1 inhibits replication, we examined whether the recombinant FHV RNA is indeed capable of replication. To this end, we introduced constructs expressing recombinant FHV RNAs into the plant Nicotiana benthamiana. Northern blot analysis of inoculated leaves suggested abundant production of recombinant FHV RNA1 and its subgenomic RNA. This demonstrated that recombinant FHV RNA with terminal extensions at both ends was competent for replication. We also showed that the recombinant FHV RNA can express the reporter gene encoding enhanced green fluorescent protein.

Keyword

Turnip yellow mosaic virus; Flock house virus; RNA replication; Reporter gene expression

MeSH Terms

Blotting, Northern
Brassica napus*
Capsid Proteins*
Genes, Reporter
Plants
RNA*
Tobacco*
Tymovirus*
Capsid Proteins
RNA