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Identification of a Novel Splice Variant (c.423-8A>G) of APC by RNA Sequencing

Kim A, Kim HK, Ahn S, Hong YS, Lim SB, Byeon JS, Chun S, Min WK, Lee W

No abstract available.
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Analysis of HCV RNA Genotypes and Quantitative Values of Korean HCV NAT Reactive Blood Donors

Shin S, Kang JW, Kang J, Seo YI, Park JR, Lee DD, Min H, Kim M

BACKGROUND: Ever since the Korean Red Cross adopted HCV NAT for blood donor screening in 2005, HCV NAT reactive donors have been identified every year. The identification of the clinical...
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Identification of Candida Species Using 26S Ribosomal RNA Gene Sequencing in Patients with Periodontitis

Al-Dabbagh NN, Al-Janabi , Al-Shuhaib MBS

The infection with Candida spp. for oral cavity is being increasingly reported. However, its variations have not yet been specifically described in periodontitis. The present study was conducted to use...
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Circular RNAs Regulate Cancer Onset and Progression via Wnt/β-Catenin Signaling Pathway

Li YF, Zhang J, Yu L

Cancer remains to be a major challenge for public health providers, and is the second leading cause of death worldwide. Therefore, it is imperative to explore the mechanisms underlying cancer...
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Rapid Whole-genome Sequencing of Zika Viruses using Direct RNA Sequencing

Kim JH, Kim J, Koo BS, Oh H, Hong JJ, Hwang ES

Zika virus (ZIKV) is one of the pathogens which is transmitted world widely, but there are no effective drugs and vaccines. Whole genome sequencing (WGS) of viruses could be applied...
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Comparison of the MGISEQ-2000 and Illumina HiSeq 4000 sequencing platforms for RNA sequencing

Jeon SA, Park JL, Kim JH, Kim JH, Kim YS, Kim JC, Kim SY

Currently, Illumina sequencers are the globally leading sequencing platform in the next-generation sequencing market. Recently, MGI Tech launched a series of new sequencers, including the MGISEQ-2000, which promise to deliver...
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Identification of neoantigens derived from alternative splicing and RNA modification

Park J, Chung YJ

The acquisition of somatic mutations is the most common event in cancer. Neoantigens expressed from genes with mutations acquired during carcinogenesis can be tumor-specific. Since the immune system recognizes tumor-specific...
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Multistaining Optimization for Epstein-Barr Virus–Encoded RNA In Situ Hybridization and Immunohistochemistry of Formalin-Fixed Paraffin-Embedded Tissues Using an Automated Immunostainer

Ko JN, Jung JK, Park YI, Shin HJ, Huh J, Back S, Kim YJ, Kim JH, Go H

BACKGROUND: Single staining is commonly performed for practical pathologic diagnoses. However, this method is limited in its ability to specify cellular morphology and immunophenotype and often requires consumption of limited...
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Analysis of Circular RNAs in the Coronary Arteries of Patients with Kawasaki Disease

Kim YK

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Circular RNA as a Possible Novel Biomarker for Kawasaki Disease

Yoon KL

No abstract available.
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Prediction of itching diagnostic marker through RNA sequencing of contact hypersensitivity and skin scratching stimulation mice models

Kim YW, Zhou T, Ko EA, Kim S, Lee D, Seo Y, Kwon N, Choi T, Lim H, Cho S, Bae G, Hwang Y, Kim D, Park H, Lee M, Jang E, Choi J, Bae H, Lim I, Bang H, Ko JH

Pruritus (itching) is classically defined as an unpleasant cutaneous sensation that leads to scratching behavior. Although the scientific criteria of classification for pruritic diseases are not clear, it can be...
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Isolation of MLL1 Inhibitory RNA Aptamers

Ul-Haq A, Jin ML, Jeong KW, Kim HM, Chun KH

Mixed lineage leukemia proteins (MLL) are the key histone lysine methyltransferases that regulate expression of diverse genes. Aberrant activation of MLL promotes leukemia as well as solid tumors in humans,...
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The Function of the Vitamin D Receptor and a Possible Role of Enhancer RNA in Epigenomic Regulation of Target Genes: Implications for Bone Metabolism

Sawatsubashi S, Nishimura , Mori J, Kouzmenko , Kato S

Vitamin D (VD) is essential for bone health, and VD or its analogues are widely used in clinics to ameliorate bone loss. The targets and mode of VD anti-osteoporotic actions...
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Small RNA sequencing profiles of mir-181 and mir-221, the most relevant microRNAs in acute myeloid leukemia

Lee YG, Kim I, Oh S, Shin DY, Koh Y, Lee KW

BACKGROUND/AIMS: To evaluate and select microRNAs relevant to acute myeloid leukemia (AML) pathogenesis, we analyzed differential microRNA expression by quantitative small RNA next-generation sequencing using duplicate marrow samples from individual...
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NFATC3–PLA2G15 Fusion Transcript Identified by RNA Sequencing Promotes Tumor Invasion and Proliferation in Colorectal Cancer Cell Lines

Jang JE, Kim HP, Han SW, Jang H, Lee SH, Song SH, Bang D, Kim TY

PURPOSE: This study was designed to identify novel fusion transcripts (FTs) and their functional significance in colorectal cancer (CRC) lines. MATERIALS AND METHODS: We performed paired-end RNA sequencing of 28 CRC...
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Ovarian Cancer Prognostic Prediction Model Using RNA Sequencing Data

Jeong S, Mok L, Kim SI, Ahn T, Song YS, Park T

Ovarian cancer is one of the leading causes of cancer-related deaths in gynecological malignancies. Over 70% of ovarian cancer cases are high-grade serous ovarian cancers and have high death rates...
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An Emerging Role for Circular RNAs in Osteoarthritis

Yu CX, Sun S

Circular RNAs (circRNAs) are currently classed as non-coding RNAs that, unlike the better known canonical linear RNAs, form a covalently closed continuous loop without 5′ or 3′ polarities. With the...
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Genetic Ablation of EWS RNA Binding Protein 1 (EWSR1) Leads to Neuroanatomical Changes and Motor Dysfunction in Mice

Yoon Y, Park H, Kim S, Nguyen PT, Hyeon SJ, Chung S, Im H, Lee J, Lee SB, Ryu H

A recent study reveals that missense mutations of EWSR1 are associated with neurodegenerative disorders such as amyotrophic lateral sclerosis, but the function of wild-type (WT) EWSR1 in the central nervous...
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RNA Binding Protein as an Emerging Therapeutic Target for Cancer Prevention and Treatment

Hong S

After transcription, RNAs are always associated with RNA binding proteins (RBPs) to perform biological activities. RBPs can interact with target RNAs in sequence- and structure-dependent manner through their unique RNA...
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An Improved Total RNA Extraction Method for White Jelly Mushroom Tremella fuciformis Rich in Polysaccharides

Zhu H, Sun X, Liu D, Zheng L, Chen L, Ma A

An improved method for extracting high quality and quantity RNA from a jelly mushroom and a dimorphic fungus—Tremella fuciformis which is especially rich in polysaccharides, is described. RNA was extracted...
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