- Imaging Gene Expression
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Lee KH
- KMID: 1991069
- Korean J Nucl Med.
- 2000 Feb;34(1):1-9.
The rapid progress of molecular genetic methods over the past two decades has necessitated the development of methods to detect and quantify genetic activity within living bodies. Reporter genes provide a rapid... -
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- Targeted Molecular Imaging
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Kim EE
- KMID: 1111248
- Korean J Radiol.
- 2003 Dec;4(4):201-210.
- doi: 10.3348/kjr.2003.4.4.201
Molecular imaging aims to visualize the cellular and molecular processes occurring in living tissues, and for the imaging of specific molecules in vivo, the development of reporter probes and dedicated... -
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- Radionuclide Reporter Gene Imaging
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Min JJ
- KMID: 2078806
- Korean J Nucl Med.
- 2004 Apr;38(2):143-151.
Recent progress in the development of non-invasive imaging technologies continues to strengthen the role of molecular imaging biological research. These tools have been validated recently in variety of research models,... -
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- Molecular Nuclear Cardiac Imaging
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Lee DS, Paeng JC
- KMID: 2078810
- Korean J Nucl Med.
- 2004 Apr;38(2):175-179.
Molecular nuclear cardiac imaging has included Tc-99m Annexin imaging to visualize myocardial apoptosis, but is now usually associated with gene therapy and cell-based therapy. Cardiac gene therapy was not successful... -
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- In Vivo Transfection of Rabbit Synovium using the Particle Bombardment Method
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Chung MS, Seong IH, Kim TG, Kim SR, Kim HJ
- KMID: 2141817
- J Korean Orthop Res Soc.
- 1998 Apr;1(1):14-22.
Treatment of arthritis with the gene-transfering technique is one of tile important applications of gene therapy in orthopedics. The synovium can be transfected with the genes producing therapeutic proteins. Among... -
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- Lactoferrin as a gene delivery vehicle to hepatocytes
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Oh ST, Rih JK, Kwon HS, Hwang DS, Kim SY, Yim JB
- KMID: 2045606
- Exp Mol Med.
- 1997 Jun;29(2):111-116.
Using lactoferrin as the specific ligand, we developed a simplified method for preparation of molecular conjugate for gene delivery. Replacement of column chromatography and dialysis by one step centrifugal filtration... -
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- Establishment of a Hepatocellular Carcinoma Cell Line Expressing Dual Reporter Genes: Sodium Iodide Symporter (NIS) and Enhanced Green Fluorescence Protein (EGFP)
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Kwak W, Koo BC, Kwon MS, Lee YJ, Lee HY, Yoo J, Kim T, Chun KS, Cheon GJ, Lee SW, Ahn BC, Lee J
- KMID: 1518945
- Nucl Med Mol Imaging.
- 2007 Jun;41(3):226-233.
PURPOSE: Dual reporter gene imaging has several advantages for more sophisticated molecular imaging studies such as gene therapy monitoring. Herein, we have constructed hepatoma cell line expressing dual reporter genes... -
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- Optical Imaging for Stem Cell Differentiation to Neuronal Lineage
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Hwang DW, Lee DS
- KMID: 1433596
- Nucl Med Mol Imaging.
- 2012 Mar;46(1):1-9.
In regenerative medicine, the prospect of stem cell therapy holds great promise for the recovery of injured tissues and effective treatment of intractable diseases. Tracking stem cell fate provides critical... -
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- A Novel Rapid Fungal Promoter Analysis System Using the Phosphopantetheinyl Transferase Gene, npgA, in Aspergillus nidulans
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Song HY, Choi D, Han DM, Kim DH, Kim JM
- KMID: 2434572
- Mycobiology.
- 2018 Dec;46(4):429-439.
- doi: 10.1080/12298093.2018.1548806
To develop a convenient promoter analysis system for fungi, a null-pigment mutant (NPG) of Aspergillus nidulans was used with the 4′-phosphopantetheinyl transferase (PPTase) gene, npgA, which restores the normal pigmentation... -
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- General Perspectives on Molecular Imaging
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Chung JK, Kang JH
- KMID: 1936464
- J Korean Med Assoc.
- 2004 Feb;47(2):112-118.
- doi: 10.5124/jkma.2004.47.2.112
Motecular imaging provides a visualization of normal as well as abnormal cellular processes at a molecular or genetic level rather than at the anatomical level. Molecular imaging is rapidly emerging... -
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- In vivo determination of the gap2 gene promoter activity in Giardia lamblia
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Yang HW, Kim J, Yong TS, Park SJ
- KMID: 1091832
- Korean J Parasitol.
- 2006 Mar;44(1):21-26.
- doi: 10.3347/kjp.2006.44.1.21
A shuttle vector for Escherichia coli and Giardia lamblia was modified to produce a reporter plasmid, which monitors the expression of prescribed gene in G. lamblia by measuring its luciferase... -
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- In Vitro Infectivity Assessment by Drug Susceptibility Comparison of Recombinant Leishmania major Expressing Enhanced Green Fluorescent Protein or EGFP-Luciferase Fused Genes with Wild-Type Parasite
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Sadeghi S, Seyed N, Etemadzadeh MH, Abediankenari S, Rafati S, Taheri T
- KMID: 2381755
- Korean J Parasitol.
- 2015 Aug;53(4):385-394.
- doi: 10.3347/kjp.2015.53.4.385
Leishmaniasis is a worldwide uncontrolled parasitic disease due to the lack of effective drug and vaccine. To speed up effective drug development, we need powerful methods to rapidly assess drug... -
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- The Role of Activation of MAP Kinase in Human Intestinal Epithelial Cells Stimulated with Bacteroides fragilis Enterotoxin
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Kim JM, Oh YK, Lee Y, Kim YJ
- KMID: 2054990
- J Bacteriol Virol.
- 2004 Mar;34(1):83-90.
A ~20 kDa heat-labile toxin (BFT) produced by enterotoxigenic B. fragilis induces chemokine responses that are associated with mucosal inflammation. In the present study, we assessed whether the activation of... -
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- Optical Imaging in the Field of Molecular Imaging
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Roh JK, Kim DE
- KMID: 1936466
- J Korean Med Assoc.
- 2004 Feb;47(2):127-132.
- doi: 10.5124/jkma.2004.47.2.127
Molecular imaging is leading an important role in the era of molecular medicine. Optical imaging, a rising star in the filed of molecular imaging, largely consists of fluorescent imaging and... -
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- Translational Imaging with PET Reporter Gene Approaches
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Min JJ
- KMID: 2139238
- Nucl Med Mol Imaging.
- 2006 Dec;40(6):279-292.
Recent progress in the development of non-invasive imaging technologies continues to strengthen the role of biomedical research. These tools have been validated recently in variety of research models, and have... -
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- Replication of Recombinant Flock House Virus RNA Encapsidated by Turnip Yellow Mosaic Virus Coat Proteins in Nicotiana benthamiana
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Kim D, Cho TJ
- KMID: 2384411
- J Bacteriol Virol.
- 2017 Jun;47(2):87-95.
- doi: 10.4167/jbv.2017.47.2.87
It was previously observed that recombinant flock house virus (FHV) RNA1 was efficiently packaged into turnip yellow mosaic virus (TYMV), provided that the TYMV coat protein (CP) sequence was present... -
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- Development of Dual Reporter System of Mutant Dopamine 2 Receptor (D2R) and Sodium Iodide Symporter (NIS) Transgenes
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Hwang DW, Lee DS, Kang JH, Chang YS, Kim YH, Jeong JM, Chung JK, Lee MC
- KMID: 1990860
- Korean J Nucl Med.
- 2004 Aug;38(4):294-299.
PURPOSE: Both human NIS and mutant D2R transgenes are proposed as reporting system in transplanted cell tracking. Using hepatoma cell lines, we constructed a dual reporter system containing human sodium-iodide... -
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- Molecular Imaging in the Age of Genomic Medicine
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Byun J
- KMID: 2166255
- Genomics Inform.
- 2007 Jun;5(2):46-55.
The convergence of molecular and genetic disciplines with non-invasive imaging technologies has provided an opportunity for earlier detection of disease processes which begin with molecular and cellular abnormalities. This emerging... -
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- Recombinant AAV Vector with MITF-M Promoter for Melanoma Gene Therapy
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Park SW, Paik SY
- KMID: 1434773
- J Bacteriol Virol.
- 2012 Mar;42(1):57-62.
- doi: 10.4167/jbv.2012.42.1.57
We have developed the recombinant adeno-associated virus (AAV) carrying the EGFP gene under the control of the microphtalmia-associated transcription factor-M (MITF-M) promoter region for melanoma-specific expression. MITF-M distal enhancer (MDE)... -
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- Introduction To Basic Molecular Biologic Techniques For Molecular Imaging Researches
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Kang JH
- KMID: 2078802
- Korean J Nucl Med.
- 2004 Apr;38(2):115-120.
Molecular imaging is a rapidly growing field due to the advances in molecular biology and imaging technologies. With the introduction of imaging reporter genes into the cell, diverse cellular processes... -
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