Korean J Physiol Pharmacol.  2017 May;21(3):279-286. 10.4196/kjpp.2017.21.3.279.

Identification of AMPK activator from twelve pure compounds isolated from Aralia Taibaiensis: implication in antihyperglycemic and hypolipidemic activities

Affiliations
  • 1Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi' an 710032, P. R. China. aidongwen71@hotmail.com miaomiaoxi02@hotmail.com
  • 2Department of Pharmacology, Chungnam National University, Daejon 34134, Korea.

Abstract

The root bark extract of Aralia taibaiensis is used traditionally for the treatment of diabetes mellitus in China. The total saponin extracted from Aralia Taibaiensis (sAT) has effective combined antihyperglycemic and hypolipidemic activities in experimental type 2 diabetic rats. However, the active compounds have not yet been fully investigated. In the present study, we examined effects of twelve triterpenoid saponins on AMP-activated protein kinase (AMPK) activation, and found that compound 28-O-β-D-glucopyranosyl ester (AT12) significantly increased phosphorylation of AMPK and Acetyl-CoA carboxylase (ACC). AT12 effectively decreased blood glucose, triglyceride (TG), free fatty acid (FFA) and low density lipoprotein-cholesterol (LDL-C) levels in the rat model of type 2 diabetes mellitus (T2DM). The mechanism by which AT12 activated AMPK was subsequently investigated. Intracellular ATP level and oxygen consumption were significantly reduced by AT12 treatment. The findings suggested AT12 was a novel AMPK activator, and could be useful for the treatment of metabolic diseases.

Keyword

AMPK; Antihyperglycemic activity; Hypolipidemic activity; T2DM; 28-O-β-D-glucopyranosyl ester (AT12)

MeSH Terms

Acetyl-CoA Carboxylase
Adenosine Triphosphate
AMP-Activated Protein Kinases*
Animals
Aralia*
Blood Glucose
China
Diabetes Mellitus
Diabetes Mellitus, Type 2
Metabolic Diseases
Models, Animal
Oxygen Consumption
Phosphorylation
Rats
Saponins
Triglycerides
AMP-Activated Protein Kinases
Acetyl-CoA Carboxylase
Adenosine Triphosphate
Blood Glucose
Saponins
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