J Korean Pediatr Soc.  1994 Sep;37(9):1226-1234.

Detection of Enterovirus in Patients with Aseptic Meningitis by Reverse Transcription and Polymerase Chain Reaction

• Yoon SJ
• Hong SJ
• Lee YH
• Kim MH
• Kim KS
• Park KH
• Lee JM
• Lee WY

Abstract

The aim of this study was to determine the applicability of reverse transcription and polymerase chain reaction (RT/PCR) for routine diagnostic use and for the detection of enteroviral mentigitis. Primers were selected in the highly conserved part of the 5'-non-coding of the enteroviral genome. Enteroviral RNA was detected by the RT/PCR in routinely collected cerebrospinal fluids (CSF) of patients with aseptic meningitis who had admitted to the dept. Of Pediatrics. MinJoong Hospital, KonKuk University from May to July 1993. Enteroviral RNA was detected in one third of CSF specimen. Our results demonstrate the significance of the RT/PCR in the diagnosis of enteroviral meningitis.

MeSH Terms

Cerebrospinal Fluid
Diagnosis
Enterovirus*
Genome
Humans
Meningitis
Meningitis, Aseptic*
Pediatrics
Polymerase Chain Reaction*
Reverse Transcription*
RNA
RNA