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Comparison Between the SFTS-QS Kit and the PowerChek SFTSV Real-time PCR Kit for the Detection of Severe Fever With Thrombocytopenia Syndrome Virus

Yoo IY, Kim JY, Yoon YK, Huh HJ, Lee NY

The recent increase in severe fever with thrombocytopenia syndrome (SFTS) cases has led to the development of the SFTS-QS kit (MiCoBioMed, Seongnam, Korea) for detecting the SFTS virus (SFTSV, now...
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False-Negative Results of Real-Time Reverse-Transcriptase Polymerase Chain Reaction for Severe Acute Respiratory Syndrome Coronavirus 2: Role of Deep-Learning-Based CT Diagnosis and Insights from Two Cases

Li D, Wang D, Dong J, Wang N, Huang H, Xu H, Xia C

The epidemic of 2019 novel coronavirus, later named as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is still gradually spreading worldwide. The nucleic acid test or genetic sequencing serves as...
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Evaluation of Multiplex Polymerase Chain Reaction Assay for the Simultaneous Detection of Sexually Transmitted Infections Using Swab Specimen

Park SH, Hwang KA, Ahn JH, Nam JH

Sexually transmitted infections (STIs) are caused by the spread of pathogens via sexual activity and can cause serious complications if left untreated, regardless of their symptoms. Therefore, early diagnosis of...
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Relationship of Circulating Cytomegalovirus Levels Obtained Through Antigenemia Testing and Quantitative PCR Differs Between Children and Adults

Choi S, Kim YJ, Yoo KH, Sung KW, Koo HH, Kim SJ, Jang JH, Kim K, Jung CW, Huh HJ, Kang ES

No abstract available.
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Performance Evaluation of the QXDx BCR-ABL %IS Droplet Digital PCR Assay

Chung HJ, Hur M, Yoon S, Hwang K, Lim HS, Kim H, Moon HW, Yun YM

Accurate detection of BCR-ABL fusion transcripts at and below molecular response (MR) 4 (0.01% International Scale [IS]) is required for disease monitoring in patients with chronic myeloid leukemia (CML). We...
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A Rapid and Effective Colony PCR Procedure for Screening Transformants in Several Common Mushrooms

Wang Y, Xu D, Liu D, Sun X, Chen Y, Zheng L, Chen L, Ma A

In the post-genomic era, gene function analysis has attracted much attention. Transformation is often needed to investigate gene function. In this study, an easy, rapid, reliable, and cost-effective colony polymerase...
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Peptoniphilus mikwangii-specific quantitative real-time polymerase chain reaction primers

Park SN, Kook JK

The purpose of this study was to develop Peptoniphilus mikwangii -specific quantitative real-time polymerase chain reaction (qPCR) primers based on the 16S ribosomal RNA (16S rDNA) gene. The specificity of...
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Detection of Streptococcus mutans in human saliva and plaque using selective media, polymerase chain reaction, and monoclonal antibodies

Lee YJ, Kim MA, Kim JG, Kim JH

The objective of this study was to evaluate the distribution of dental caries-associated Streptococcus mutans in human saliva and plaque. The samples for this study were collected from 90 subjects...
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Comparison of Multiplex Real-Time Polymerase Chain Reaction Assays for Detection of Respiratory Viruses in Nasopharyngeal Specimens

Uwizeyimana JD, Kim MK, Kim D, Byun JH, Yong D

BACKGROUND: Respiratory tract infections are major public health threats, and the identification of their causative microbes helps clinicians to initiate timely and appropriate antimicrobial therapy and prevent the secondary spread...
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Multiplex PCR analysis of virulence genes and their influence on antibiotic resistance in Enterococcus spp. isolated from broiler chicken

Song H, Bae Y, Jeon E, Kwon Y, Joh S

Enterococcus spp. are opportunistic pathogens that cause lameness in broiler chickens, resulting in serious economic losses worldwide. Virulence of Enterococcus spp. is associated with several putative virulence genes including fsr,...
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Comparison of the Effectiveness in the Application of Competitive and Noncompetitive Internal Control for the Laboratory Developed Polymerase Chain Reaction

Shin S, Kang JW, Kang JW, Seo YI, Min H

BACKGROUND: A nucleic acid amplification test was adopted to detect transfusion-transmitted infectious agents. In the case of HTLV, however, there was no internal control (IC) because the laboratory developed polymerase...
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The impact of corticosteroid use on inpatients with inflammatory bowel disease and positive polymerase chain reaction for Clostridium difficile

Lim HW, Schuster I, Rajapakse , Monzur F, Khan S, Sultan K

BACKGROUND/AIMS: Optimal management of inflammatory bowel disease (IBD) with concomitant Clostridium difficile infection (CDI) is controversial, especially when CDI diagnosis is made by polymerase chain reaction (PCR) testing, which may...
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A Case of Cutaneous Non-Mycobacterium Infection after Illegal Botulinum Toxin Injection Diagnosed by Polymerase Chain Reaction

Jung MJ, Chung BY, Choi YW, Son JH, Kim HO, Park CW

No abstract available.
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Optimization of ultra-fast convection polymerase chain reaction conditions for pathogen detection with nucleic acid lateral flow immunoassay

Kim TH, Hwang HJ, Kim JH

Recently, the importance of on-site detection of pathogens has drawn attention in the field of molecular diagnostics. Unlike in a laboratory environment, on-site detection of pathogens is performed under limited...
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Development and Evaluation of Multiplex PCR for the Detection of Carbapenemase-Producing Enterobacteriaceae

Kim SH, Bae IK, Kim NY, Song SA, Kim S, Jeong J, Shin JH

BACKGROUND: The isolation of carbapenemase-producing Enterobacteriaceae (CPE) has become increasingly common. Continuous surveillance for these organisms is essential because their infections are closely related to outbreaks of illness and are...
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Comparative Evaluation Between the RealStar Pneumocystis jirovecii PCR Kit and the AmpliSens Pneumocystis jirovecii (carinii)-FRT PCR Kit for Detecting P. jirovecii in Non-HIV Immunocompromised Patients

Huh HJ, Lim KR, Ki CS, Huh K, Shim HJ, Song DJ, Kim YJ, Chung DR, Lee NY

BACKGROUND: Real-time PCR is more sensitive than microscopic examination for detecting Pneumocystis jirovecii. We compared the performance of two assays for detecting P. jirovecii DNA: the RealStar Pneumocystis jirovecii PCR...
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Comparison of Two PCR Assays for Trichomonas vaginalis

Noh CS, Kim SS, Park SY, Moon HS, Hong Y, Ryu JS

PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in...
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Diagnostic Utility of Polymerase Chain Reaction for Acanthamoeba in Contact Lens-related Keratitis with Epithelial Defects

Kang MC, Lim DH, Huh HJ, Yoo IY, Chung TY

PURPOSE: To report contact lens related Acanthamoeba keratitis with corneal epithelial defect cases which were diagnosed using polymerase chain reaction (PCR). CASE SUMMARY: A 51-year-old male visited our hospital for loss...
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Molecular Genetics of von Willebrand Disease in Korean Patients: Novel Variants and Limited Diagnostic Utility of Multiplex Ligation-Dependent Probe Amplification Analyses

Kim HJ, Kim SK, Yoo KY, Lee KO, Yun JW, Kim SH, Kim HJ, Park SK

BACKGROUND: von Willebrand disease (VWD), characterized by quantitative or qualitative defects of von Willebrand factor (VWF), is the most common inheritable bleeding disorder. Data regarding the genetic background of VWD...
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A Case of Tuberculosis Verrucosa Cutis Confirmed by Detection of Mycobacterium tuberculosis DNA Using by Polymerase Chain Reaction

Kim KS, Yang SY, Hahm JE, Kim SS, Kim CW

  • KMID: 2433653
  • Korean J Dermatol.
  • 2019 Jan;57(1):57-58.
No abstract available.
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