Nutr Res Pract.  2015 Jun;9(3):227-234. 10.4162/nrp.2015.9.3.227.

Dual effects of a mixture of grape pomace (Campbell Early) and Omija fruit ethanol extracts on lipid metabolism and the antioxidant defense system in diet-induced obese mice

• Han HJ
• Jung UJ
• Kim HJ
• Moon BS
• Cho SJ
• Park YB
• Lee DG
• Choi MS

• KMID: 2313834
• DOI: http://doi.org/10.4162/nrp.2015.9.3.227

Abstract

BACKGROUND/OBJECTIVES
We investigated the effects of a combination of grape pomace (Vitis labrusca, Campbell Early) and Omija fruit (Schizandra chinensis, Baillon) ethanol extracts on lipid metabolism and antioxidant defense system in diet-induced obese mice.
MATERIALS/METHODS
Forty male C57BL/6J mice were divided into four groups and fed high-fat diet (control group, CON) or high-fat diet added 0.5% grape pomace extract (GPE), 0.05% Omija fruit extract (OFE) or 0.5% GPE plus 0.05% OFE (GPE+OFE) for 12 weeks.
RESULTS
In contrast to the GPE- or OFE-supplemented groups, the GPE+OFE group showed significantly lower body weight and white adipose tissue weights than the CON group. Moreover, GPE+OFE supplementation significantly decreased plasma total cholesterol and increased the plasma HDL-cholesterol/total-cholesterol ratio (HTR) compared to the control diet. The hepatic triglyceride level was significantly lower in the GPE+OFE and GPE groups by increasing beta-oxidation and decreasing lipogenic enzyme compared to the CON group. Furthermore, GPE+OFE supplementation significantly increased antioxidant enzyme activities with a simultaneous decrease in liver H2O2 content compared to the control diet.
CONCLUSIONS
Together our results suggest that supplementation with the GPE+OFE mixture may be more effective in improving adiposity, lipid metabolism and oxidative stress in high-fat diet-fed mice than those with GPE and OFE alone.

Keyword

Grape pomace; Omija fruit; high-fat diet; lipid-regulating enzyme; antioxidant enzyme

MeSH Terms

Adipose Tissue, White
Adiposity
Animals
Body Weight
Cholesterol
Diet
Diet, High-Fat
Ethanol*
Fruit*
Humans
Lipid Metabolism*
Liver
Male
Mice
Mice, Obese*
Oxidative Stress
Plasma
Triglycerides
Vitis*
Weights and Measures
Cholesterol
Ethanol